从轮叶蒲桃（桃金娘科）叶提取物中分离鉴定具有生物活性的抗菌成分。
Isolation and identification of bioactive antibacterial components in leaf extracts of Vangueria spinosa (Rubiaceae).
机构信息
Mosquito and Microbiology Research Units, Parasitology Laboratory, Department of Zoology, The University of Burdwan West Bengal, India.
出版信息
Asian Pac J Trop Med. 2011 Jan;4(1):35-40. doi: 10.1016/S1995-7645(11)60028-X. Epub 2011 Jan 15.
OBJECTIVE
The column chromatographic fraction of ethyl acetate (EA1, EA2, EA3, EA4 and EA5) leaf extracts of Vangueria spinosa (V. spinosa) were screened for antibacterial activity and phytochemical analysis.
METHODS
EA3 fraction was isolated and identified by column chromatography, thin layer chromatography, spectral data analysis and phytochemical screening were used for analysis.
RESULTS
EA3 fraction was significantly active at 4 to 64 mg/L against Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa with minimum inhibitory concentration of 1.5625 to 3.1250 mg/mL. The active fraction (EA3) revealed the presence of flavonoid with retention factor value (R(f)) of 0.39. The active antibacterial agent in the most potent fraction (EA3) was isolated and identified as flavonoid (-)-epicatechin-3-O-β-glucopyranoside by thin layer chromatography (TLC) and phytochemical screening. EA1 and EA2 show inhibitory activity at 4 to 64 mg/L against Staphylococcus aureus only where as fraction EA4 and EA5 do not shows any inhibitory activity within that range of concentration against any bacteria.
CONCLUSIONS
The results support the ethnomedicinal use of leaf of V. spinosa for the treatment of bacterial diseases.
目的
对蓬虆（Vangueria spinosa）叶乙酸乙酯（EA1、EA2、EA3、EA4 和 EA5）萃取物的柱层析部分进行筛选，以评估其抗菌活性和进行植物化学分析。
方法
通过柱层析、薄层色谱、光谱数据分析和植物化学筛选对 EA3 部分进行分离和鉴定。
结果
EA3 部分在 4 至 64mg/L 浓度下对金黄色葡萄球菌、大肠杆菌、肺炎克雷伯菌和铜绿假单胞菌表现出显著的活性，最小抑菌浓度为 1.5625 至 3.1250mg/mL。活性部分（EA3）显示存在黄酮类化合物，保留因子值（R(f)）为 0.39。在最有效的部分（EA3）中分离出的活性抗菌剂被鉴定为黄酮类化合物（-）-表儿茶素-3-O-β-吡喃葡萄糖苷，通过薄层色谱（TLC）和植物化学筛选进行鉴定。EA1 和 EA2 仅在 4 至 64mg/L 浓度下对金黄色葡萄球菌表现出抑制活性，而 EA4 和 EA5 在该浓度范围内对任何细菌均无抑制活性。
结论
这些结果支持了蓬虆叶用于治疗细菌病的传统医学用途。
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