基于适体的靶标/探针介导的循环信号放大技术对肺癌细胞中表皮生长因子受体的高灵敏度检测
Highly sensitive detection of epidermal growth factor receptor in lung cancer cells by aptamer-based target-/probe-mediated cyclic signal amplification.
作者信息
Zhang Dandan, Ma Fei, Zhang Qianyi, Zhang Chun-Yang
机构信息
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China.
出版信息
Chem Commun (Camb). 2017 Oct 17;53(83):11496-11499. doi: 10.1039/c7cc06823a.
We develop an antibody-free fluorescence method for the epidermal growth factor receptor (EGFR) assay using aptamer-based target-/probe-mediated cyclic signal amplification. The method is highly sensitive with a detection limit of 0.16 fM, and it can be applied to detect EGFR in lung cancer cells, holding great potential in clinical diagnosis.
我们开发了一种用于表皮生长因子受体（EGFR）检测的无抗体荧光方法，该方法使用基于适体的靶标/探针介导的循环信号放大技术。该方法具有高灵敏度，检测限为0.16 fM，可用于检测肺癌细胞中的EGFR，在临床诊断中具有巨大潜力。
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