Division of Mechanical and Biomedical Engineering, Ewha Womans University , Seoul 03760, South Korea.
Department of Materials Science and Engineering, Stanford University , Stanford, California 94305, United States.
Nano Lett. 2017 Nov 8;17(11):6644-6652. doi: 10.1021/acs.nanolett.7b02591. Epub 2017 Oct 20.
Each immunoglobulin isotype has unique immune effector functions. The contribution of these functions in the elimination of pathogens and tumors can be determined by monitoring quantitative temporal changes in isotype levels. Here, we developed a novel technique using magneto-nanosensors based on the effect of giant magnetoresistance (GMR) for longitudinal monitoring of total and antigen-specific isotype levels with high precision, using as little as 1 nL of serum. Combining in vitro serologic measurements with in vivo imaging techniques, we investigated the role of the antibody response in the regression of firefly luciferase (FL)-labeled lymphoma cells in spleen, kidney, and lymph nodes in a syngeneic Burkitt's lymphoma mouse model. Regression status was determined by whole body bioluminescent imaging (BLI). The magneto-nanosensors revealed that anti-FL IgG2a and total IgG2a were elevated and sustained in regression mice compared to non-regression mice (p < 0.05). This platform shows promise for monitoring immunotherapy, vaccination, and autoimmunity.
每种免疫球蛋白同种型都具有独特的免疫效应功能。通过监测同种型水平的定量时间变化，可以确定这些功能在清除病原体和肿瘤中的作用。在这里，我们开发了一种使用基于巨磁电阻 (GMR) 效应的磁纳米传感器的新技术，用于通过纵向监测总同种型和抗原特异性同种型水平，具有高精度，使用的血清量少至 1 nL。我们将体外血清学测量与体内成像技术相结合，研究了抗体反应在同种异体 Burkitt 淋巴瘤小鼠模型中脾脏、肾脏和淋巴结中萤火虫荧光素酶 (FL) 标记淋巴瘤细胞消退中的作用。通过全身生物发光成像 (BLI) 确定消退状态。与非消退小鼠相比，磁纳米传感器显示出抗-FL IgG2a 和总 IgG2a 在消退小鼠中升高并持续存在（p < 0.05）。该平台有望用于监测免疫疗法、疫苗接种和自身免疫。
