评估网织红细胞血红蛋白含量（RET-He）在犬缺铁性红细胞生成诊断中的作用。

Evaluation of reticulocyte hemoglobin content (RET-He) in the diagnosis of iron-deficient erythropoiesis in dogs.

作者信息

Fuchs Jannika, Moritz Andreas, Grußendorf Esther, Lechner Jörg, Neuerer Felix, Nickel Rafael, Rieker Thomas, Schwedes Claudia, DeNicola Dennis B, Russell James, Bauer Natali

机构信息

Department of Veterinary Clinical Sciences, Clinical Pathology and Clinical Pathophysiology, Justus-Liebig-University, Giessen, Germany.

Small Animal Clinic Grußendorf, Bramsche, Germany.

出版信息

Vet Clin Pathol. 2017 Dec;46(4):558-568. doi: 10.1111/vcp.12547. Epub 2017 Oct 9.

DOI:10.1111/vcp.12547

PMID:28991391

Abstract

BACKGROUND

Reticulocyte hemoglobin content provided by the Siemens ADVIA (CHr) is an established marker of iron deficiency. The IDEXX ProCyte Dx hematology analyzer now provides a similar variable, reticulocyte hemoglobin equivalent (RET-He).

OBJECTIVES

The objective was to evaluate RET-He and its diagnostic utility in dogs, and to calculate a cutoff value for diagnosing iron-deficient erythropoiesis (IDE). Furthermore, the prevalence of RET-He values below this cutoff value was established.

METHODS

One hundred and seventy-one CBCs of healthy dogs were used to establish a RI. Stability of RET-He was evaluated by repeated measurements over 48 hours (n = 10). The 25-run coefficient of variation (CV) was calculated, and correlation and bias between measurements of RET-He and CHr were assessed (n = 190). A cutoff value for diagnosing IDE was calculated. The utility of RET-He in the detection of IDE was evaluated in 123 dogs. The prevalence of low RET-He values was assessed retrospectively in a multicenter study (2012-2014) under participation of 7 veterinary clinics.

RESULTS

Reticulocyte hemoglobin equivalent with an RI of 22.2 to 28.6 pg was statistically stable over 48 hours (P = .10). The CV was 1.8%. A fair correlation (ρ = 0.74) between RET-He and CHr with a small bias of -0.6 pg was found. The cutoff value for diagnosing IDE was 20.9 pg (sensitivity: 85%; specificity: 99%). The prevalence of RET-He values below 20.9 pg was 10.3% (1084/10,553 dogs).

CONCLUSIONS

RET-He on the ProCyte Dx is a precise screening tool in dogs to detect iron-deficient erythropoiesis.

摘要

背景

西门子ADVIA提供的网织红细胞血红蛋白含量（CHr）是缺铁的既定标志物。IDEXX ProCyte Dx血液分析仪现在提供了一个类似的变量，即网织红细胞血红蛋白当量（RET-He）。

目的

目的是评估RET-He及其在犬类中的诊断效用，并计算诊断缺铁性红细胞生成（IDE）的临界值。此外，还确定了低于该临界值的RET-He值的患病率。

方法

使用171份健康犬的全血细胞计数（CBC）来建立参考区间（RI）。通过在48小时内重复测量来评估RET-He的稳定性（n = 10）。计算25次运行的变异系数（CV），并评估RET-He和CHr测量之间的相关性和偏差（n = 190）。计算诊断IDE的临界值。在123只犬中评估RET-He在检测IDE中的效用。在一项由7家兽医诊所参与的多中心研究（2012 - 2014年）中，回顾性评估低RET-He值的患病率。

结果

网织红细胞血红蛋白当量的参考区间为22.2至28.6 pg，在48小时内具有统计学稳定性（P = 0.10）。CV为1.8%。发现RET-He与CHr之间具有中等相关性（ρ = 0.74），偏差较小，为 - 0.6 pg。诊断IDE的临界值为20.9 pg（敏感性：85%；特异性：99%）。低于20.9 pg的RET-He值的患病率为10.3%（1084/10553只犬）。