Small Animal Clinic, Internal Medicine, Justus-Liebig-University, Giessen, Germany.
Department of Veterinary Clinical Sciences, Clinical Pathology and Clinical Pathophysiology, Justus-Liebig-University, Giessen, Germany.
Vet Clin Pathol. 2020 Dec;49(4):557-566. doi: 10.1111/vcp.12925.
Decreased reticulocyte hemoglobin content (CHr) (Siemens ADVIA 2120) reflects iron-limited erythropoiesis (ILE). RETIC-HGB (IDEXX ProCyte Dx) is a novel marker of ILE for veterinary use.
We aimed to evaluate reference intervals (RIs) and the utility of RETIC-HGB and CHr in the diagnosis of feline ILE.
RIs were established in 59 healthy cats. Intra-assay coefficients of variation (CVs) and correlations between RETIC-HGB and CHr were assessed. Two hundred and seventy-five cats were classified as having ILE or not based on low plasma iron or low transferrin saturation along with anemia and/or altered RBC indices. CHr, RETIC-HGB, and serum amyloid A (SAA) were compared between the groups. The sensitivity and specificity of RETIC-HGB and CHr to diagnose ILE were analyzed to determine the RI lower limits.
RIs for RETIC-HGB and CHr were 12.5-18.0 and 14.0-19.9 pg, respectively. The CV was 3% for both variables. RETIC-HGB and CHr were moderately correlated (r = 0.59) with a bias of -1.2 picograms (pgs). Twenty of the 275 cats were classified as having ILE. Compared with non-ILE cats, ILE cats had significantly lower median RETIC-HGB (14.3 vs 15.2 pg, P = .0046) and mean CHr (14.7 vs 16.5 pg, P < .0001) values and significantly increased median SAA (44.6 vs 2.3 µg/dl, P < .0001) values. Using the lower RI limits resulted in a low sensitivity and relatively high specificity to diagnose ILE in cats.
ILE was characterized by decreased CHr and RETIC-HGB; however, sensitivity was low. The moderate correlation between RETIC-HGB and CHr is likely due to species differences and different methodology.
网织红细胞血红蛋白含量（CHr）降低（西门子 ADVIA 2120）反映了铁限性红细胞生成（ILE）。REtic-HGB（IDEXX ProCyte Dx）是一种用于兽医的新型 ILE 标志物。
我们旨在评估健康猫的参考区间（RI）和 RETIC-HGB 和 CHr 在猫 ILE 诊断中的应用。
在 59 只健康猫中建立了 RI。评估了日内变异系数（CV）和 RETIC-HGB 与 CHr 之间的相关性。根据低血浆铁或低转铁饱和度以及贫血和/或改变的 RBC 指数，将 275 只猫分为 ILE 或非 ILE。比较两组间的 CHr、REtic-HGB 和血清淀粉样蛋白 A（SAA）。分析 RETIC-HGB 和 CHr 诊断 ILE 的敏感性和特异性，以确定 RI 的下限。
REtic-HGB 和 CHr 的 RI 分别为 12.5-18.0 和 14.0-19.9 pg。两种变量的 CV 均为 3%。REtic-HGB 和 CHr 中度相关（r=0.59），偏差为-1.2 皮克（pg）。275 只猫中有 20 只被归类为 ILE。与非 ILE 猫相比，ILE 猫的中位 RETIC-HGB（14.3 比 15.2 pg，P=0.0046）和平均 CHr（14.7 比 16.5 pg，P<0.0001）值显著降低，中位 SAA（44.6 比 2.3 µg/dl，P<0.0001）值显著升高。使用 RI 下限会导致诊断猫 ILE 的敏感性低而特异性相对较高。
ILE 的特点是 CHr 和 RETIC-HGB 降低；然而，敏感性较低。REtic-HGB 和 CHr 之间的中度相关性可能是由于物种差异和不同的方法学所致。
