Division of Internal Medicine, University Hospital Basel, and Clinical Immunology Lab, Department of Biomedicine, University of Basel, Switzerland.
Department of Nephrology, University Hospital Essen, University Duisburg-Essen, Germany.
Nephrol Dial Transplant. 2018 Jan 1;33(1):54-64. doi: 10.1093/ndt/gfx220.
Levels of serum BAFF, IgG anti-BAFF and BAFF-IgG complexes were quantified by enzyme-linked immunosorbent assay. IgG anti-BAFF and BAFF-IgG complexes were further characterized using serum fractions obtained by fast protein liquid chromatography. To study the association of serum BAFF, IgG anti-BAFF and BAFF-IgG complex levels with SLE manifestations, 373 visits from 178 patients prospectively included in the Swiss SLE Cohort Study were analysed.
While IgG anti-BAFF levels were not associated with clinical manifestations of SLE, serum BAFF levels correlated with disease activity and were higher in patients with renal involvement. Interestingly, we could also demonstrate the occurrence of BAFF-IgG complexes of different sizes in the sera of SLE patients, which were not due to treatment with belimumab and differed from complexes constructed in vitro. Most strikingly, the levels of these BAFF-IgG complexes were found to strongly correlate with overall disease activity, low complement levels and a history of lupus nephritis.
BAFF-IgG complexes strongly correlate with disease activity in SLE patients, suggesting a pathogenic role in SLE.
通过酶联免疫吸附试验定量测定血清 BAFF、IgG 抗 BAFF 和 BAFF-IgG 复合物的水平。使用快速蛋白液相色谱获得的血清级分进一步表征 IgG 抗 BAFF 和 BAFF-IgG 复合物。为了研究血清 BAFF、IgG 抗 BAFF 和 BAFF-IgG 复合物水平与 SLE 表现的相关性，前瞻性纳入瑞士 SLE 队列研究的 178 例患者的 373 次就诊进行了分析。
虽然 IgG 抗 BAFF 水平与 SLE 的临床表现无关，但血清 BAFF 水平与疾病活动度相关，且在有肾脏受累的患者中更高。有趣的是，我们还可以在 SLE 患者的血清中证明存在不同大小的 BAFF-IgG 复合物，这些复合物不是由于使用贝利木单抗引起的，也不同于体外构建的复合物。最引人注目的是，这些 BAFF-IgG 复合物的水平与疾病的总体活动度、低补体水平和狼疮肾炎病史强烈相关。
BAFF-IgG 复合物与 SLE 患者的疾病活动度密切相关，提示其在 SLE 中具有致病作用。
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