Division of Internal Medicine and Clinical Immunology laboratory, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland.
Department of Nephrology, University Hospital, Essen, Germany.
Front Immunol. 2018 Oct 2;9:2090. doi: 10.3389/fimmu.2018.02090. eCollection 2018.
Autoantibodies and aberrant immune complexes are pathological hallmarks of systemic lupus erythematosus (SLE). This study aimed to determine the occurrence of IgG autoantibodies against human serum albumin (anti-HSA IgG) and their potential association with antibodies against bovine serum albumin (anti-BSA IgG) in patients with SLE. Sera of 180 SLE patients included to the Swiss SLE Cohort Study and 188 age- and sex-matched healthy controls were evaluated. Levels of anti-HSA IgG and anti-BSA IgG were quantified by ELISA. Selected samples were further characterized using serum fractions obtained by fast liquid chromatography (FPLC). SLE patients had increased levels of anti-HSA IgG ( = 0.002) but similar levels of anti-BSA IgG compared to matched healthy controls. Anti-HSA IgG levels correlated with the SLE Disease Activity Index (SLEDAI), which was more pronounced in patients with an physician's global assessment (PGA) of ≥ 1 ( = 0.309, = 0.0066). Anti-HSA IgG was partially complexed with serum albumin but also occurred as monomeric autoantibodies in highly positive SLE patients. A positive correlation between anti-HSA IgG and anti-BSA IgG was found that was stronger in SLE patients than in healthy controls ( = 0.3172, < 0.001 vs. = 0.2122, < 0.0035). Binding of anti-BSA IgG was inhibited partially in the presence of HSA in samples with double positivity for anti-HSA and anti-BSA (median inhibition 47.9%, range 0.9-100%) and vice versa. In SLE patients there is an increased prevalence of anti-HSA IgG antibodies that are associated with SLE disease activity.
自身抗体和异常免疫复合物是系统性红斑狼疮（SLE）的病理标志。本研究旨在确定针对人血清白蛋白（抗-HSA IgG）的 IgG 自身抗体的发生及其与 SLE 患者中针对牛血清白蛋白（抗-BSA IgG）的抗体的潜在关联。
评估了瑞士 SLE 队列研究中的 180 例 SLE 患者和 188 名年龄和性别匹配的健康对照者的血清。通过酶联免疫吸附试验（ELISA）定量测定抗-HSA IgG 和抗-BSA IgG 的水平。使用快速液相色谱（FPLC）获得的血清级分进一步表征选定的样品。
与匹配的健康对照者相比，SLE 患者具有更高水平的抗-HSA IgG（ = 0.002），但抗-BSA IgG 水平相似。抗-HSA IgG 水平与 SLE 疾病活动指数（SLEDAI）相关，在 PGA≥1 的患者中更为明显（ = 0.309， = 0.0066）。抗-HSA IgG 部分与血清白蛋白复合，但在高度阳性的 SLE 患者中也作为单体自身抗体存在。发现抗-HSA IgG 与抗-BSA IgG 之间存在正相关，在 SLE 患者中比在健康对照者中更强（ = 0.3172， < 0.001 与 = 0.2122， < 0.0035）。在抗-HSA 和抗-BSA 双阳性样本中，存在 HSA 时，抗-BSA IgG 的结合部分被抑制（中位数抑制率为 47.9％，范围为 0.9-100％），反之亦然。
在 SLE 患者中，抗-HSA IgG 抗体的患病率增加，与 SLE 疾病活动相关。
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