Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347; email:
Annu Rev Cell Dev Biol. 2017 Oct 6;33:1-22. doi: 10.1146/annurev-cellbio-100616-060827.
During my graduate work with Keith Porter, I became fascinated by the mitotic spindle, an interest that has motivated much of my scientific work ever since. I began spindle studies by using electron microscopes, instruments that have made significant contributions to our understanding of spindle organization. Such instruments have helped to elucidate the distributions of spindle microtubules, the interactions among them, their molecular polarity, and their associations with both kinetochores and spindle poles. Our lab has also investigated some processes of spindle physiology: microtubule dynamics, the actions of microtubule-associated proteins (including motor enzymes), the character of forces generated by specific spindle components, and factors that control mitotic progression. Here, I give a personal perspective on some of this intellectual history and on what recent discoveries imply about the mechanisms of chromosome motion.
在与 Keith Porter 合作研究生工作期间，我对有丝分裂纺锤体产生了浓厚的兴趣，这一兴趣一直激励着我从事此后的大部分科学工作。我开始通过使用电子显微镜研究纺锤体，电子显微镜的应用对我们理解纺锤体的结构做出了重大贡献。这些仪器帮助阐明了纺锤体微管的分布、它们之间的相互作用、它们的分子极性以及它们与着丝粒和纺锤极的关联。我们实验室还研究了一些纺锤体生理学过程：微管动力学、微管相关蛋白的作用（包括马达酶）、特定纺锤体成分产生的力的性质，以及控制有丝分裂进程的因素。在这里，我从个人角度介绍了这方面的一些历史，并讨论了最近的发现对染色体运动机制的意义。
