Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 60965 Poznan, Poland.
Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 60965 Poznan, Poland.
Sci Total Environ. 2018 Feb 15;615:784-795. doi: 10.1016/j.scitotenv.2017.09.213. Epub 2017 Oct 7.
Due to the rapid growth in quantities of phenolic compounds in wastewater, the development of efficient and environmentally friendly methods for their removal becomes a necessity. Thus, in a presented work, for the first time, a novel material, Hippospongia communis spongin-based scaffold, was used as a biopolymeric support for the immobilization of laccase from Trametes versicolor. The resulting biocatalytic systems were used for the biodegradation of three bisphenols: bisphenol A (BPA), bisphenol F (BPF) and bioremoval-resistant bisphenol S (BPS). Optimization of the immobilization and biodegradation methodologies was performed to increase bisphenols removal. The effect of temperature, pH and initial pollutant concentration was evaluated. It was shown that under optimal conditions, almost 100% of BPA (pH5, 30°C) and BPF (pH5, 40°C), and over 40% of BPS (pH4, 30°C) was removed from the solution at a concentration of 2mg/mL. Furthermore, the immobilized laccase exhibited good reusability and storage stability, retaining over 80% of its initial activity after 50days of storage. In addition, the main biodegradation products of BPA and BPF were identified. It was shown that mainly dimers and trimers were formed following the oxidation of bisphenols by the immobilized laccase.
由于废水中酚类化合物的数量迅速增加，因此开发高效、环保的去除方法成为必要。因此，在本工作中，首次将新型材料——海绵 Hippospongia communis 海绵蛋白骨架用作固定化漆酶的生物聚合物载体，该漆酶来自于变色栓菌。所得的生物催化体系用于三种双酚的生物降解：双酚 A（BPA）、双酚 F（BPF）和生物去除抗性双酚 S（BPS）。优化了固定化和生物降解方法，以提高双酚类物质的去除率。评估了温度、pH 值和初始污染物浓度的影响。结果表明，在最佳条件下，在 2mg/mL 的浓度下，几乎 100%的 BPA（pH5，30°C）和 BPF（pH5，40°C），以及超过 40%的 BPS（pH4，30°C）可以从溶液中去除。此外，固定化漆酶表现出良好的可重复使用性和储存稳定性，在储存 50 天后仍保留超过 80%的初始活性。此外，还鉴定了 BPA 和 BPF 的主要生物降解产物。结果表明，在固定化漆酶氧化双酚类物质后，主要形成二聚体和三聚体。
