Salgueiro Rafael Barrera, Gerlinger-Romero Frederico, Guimarães-Ferreira Lucas, de Castro Barbosa Thais, Nunes Maria Tereza
Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, 05508-900 São Paulo, Sao Paulo, Brazil.
School of Exercise and Nutrition Sciences, Institute for Physical Activity and Nutrition (IPAN), Deakin University, VIC, Australia.
Life Sci. 2017 Dec 15;191:17-23. doi: 10.1016/j.lfs.2017.10.001. Epub 2017 Oct 7.
L-Arginine has emerged as an important supplement for athletes and non-athletes in order to improve performance. Arginine has been extensively used as substrate for nitric oxide synthesis, leading to increased vasodilatation and hormonal secretion. However, the chronic consumption of arginine has been shown to impair insulin sensitivity. In the present study, we aimed to evaluate whether chronic arginine supplementation associated with exercise training would have a beneficial impact on insulin sensitivity. We, therefore, treated Wistar rats for 4weeks with arginine, associated or not with exercise training (treadmill). We assessed the somatotropic activation, by evaluating growth hormone (GH) gene expression and protein content in the pituitary, as well is GH concentration in the serum. Additionally, we evaluate whole-body insulin sensitivity, by performing an insulin tolerance test. Skeletal muscle morpho-physiological parameters were also assessed. Insulin sensitivity was impaired in the arginine-treated rats. However, exercise training reversed the negative effects of arginine. Arginine and exercise training increased somatotropic axis function, muscle mass and body weight gain. The combination arginine and exercise training further decreased total fat mass. Our results confirm that chronic arginine supplementation leads to insulin resistance, which can be reversed in the association with exercise training. We provide further evidence that exercise training is an important tool to improve whole-body metabolism.
L-精氨酸已成为运动员和非运动员提高运动表现的重要补充剂。精氨酸已被广泛用作一氧化氮合成的底物，导致血管舒张和激素分泌增加。然而，长期摄入精氨酸已被证明会损害胰岛素敏感性。在本研究中，我们旨在评估长期补充精氨酸并结合运动训练是否会对胰岛素敏感性产生有益影响。因此，我们用精氨酸对Wistar大鼠进行了4周的治疗，同时进行或不进行运动训练（跑步机运动）。我们通过评估垂体中生长激素（GH）的基因表达和蛋白质含量以及血清中的GH浓度来评估生长激素轴的激活情况。此外，我们通过进行胰岛素耐量试验来评估全身胰岛素敏感性。我们还评估了骨骼肌的形态生理参数。精氨酸处理的大鼠胰岛素敏感性受损。然而，运动训练逆转了精氨酸的负面影响。精氨酸和运动训练增加了生长激素轴功能、肌肉质量和体重增加。精氨酸与运动训练相结合进一步降低了总脂肪量。我们的结果证实，长期补充精氨酸会导致胰岛素抵抗，而运动训练可以逆转这种抵抗。我们进一步证明，运动训练是改善全身代谢的重要手段。
