采用 GenoType MTBDRplus assay 分析摩洛哥结核分枝杆菌分离株中的异烟肼和利福平耐药性。

Analysis of isoniazid and rifampicin resistance in Mycobacterium tuberculosis isolates in Morocco using GenoType MTBDRplus assay.

机构信息

Laboratory of Microbiology and Molecular Biology, Faculty of Sciences, Mohammed V University, Rabat, Morocco; National Tuberculosis Reference Laboratory, National Institute of Hygiene, Rabat, Morocco.

National Tuberculosis Reference Laboratory, National Institute of Hygiene, Rabat, Morocco.

出版信息

J Glob Antimicrob Resist. 2018 Mar;12:197-201. doi: 10.1016/j.jgar.2017.09.017. Epub 2017 Oct 7.

DOI:10.1016/j.jgar.2017.09.017

PMID:28993300

Abstract

OBJECTIVES

The number of multidrug-resistant tuberculosis (MDR-TB) cases is rising worldwide. The present investigation aimed to evaluate, using the GenoType MTBDRplus assay, the most common mutations associated with rifampicin (RIF) and isoniazid (INH) resistance among resistant strains in Morocco.

METHODS

A total of 319 Mycobacterium tuberculosis isolates sent to the National Tuberculosis Reference Laboratory between 2013 and 2015 were subjected to GenoType MTBDRplus for detecting M. tuberculosis and determination of drug susceptibility. Correlation of the minimum inhibitory concentrations (MICs) of INH with genotypic assay results was carried out for 97 MDR-TB strains. Various concentrations of INH were tested.

RESULTS

The most frequent mutations observed were rpoBS531L (67.2%) and katGS315T1/2 (66.5%). Isolates with inhA gene mutation, katG gene mutation, and dual mutations in katG and inhA had MICs ranging from 0.5-1μg/mL, 2-10μg/mL and ≥12μg/mL, respectively.

CONCLUSION

In Morocco, 66.5% and 76.7% of M. tuberculosis strains carried mutations causing high-level resistance to INH and RIF, respectively.

摘要

目的

全球耐多药结核病（MDR-TB）病例数量不断增加。本研究旨在使用 GenoType MTBDRplus 检测在摩洛哥分离的耐药菌株中与利福平（RIF）和异烟肼（INH）耐药相关的最常见突变。

方法

2013 年至 2015 年期间，共将 319 株结核分枝杆菌分离株送至国家结核病参考实验室进行 GenoType MTBDRplus 检测，以检测结核分枝杆菌并确定药物敏感性。对 97 株耐多药结核分枝杆菌菌株进行了 INH 基因型检测结果与最低抑菌浓度（MIC）的相关性分析。测试了不同浓度的 INH。