Kontny I, Thielebeule U
Bezirks-Hygieneinspektion und -institut Rostock.
Zentralbl Bakteriol Mikrobiol Hyg B Umwelthyg Krankenhaushyg Arbeitshyg Prav Med. 1988 Mar;186(1):55-66.
262 samples of river water had been analysed for the occurrence of antibiotic resistant Aeromonas and Vibrio strains. The bacteria were cultured on selective media containing the antimicrobial chemotherapeutic agents oxytetracycline, chloramphenicol, streptomycin, ampicillin, kanamycin, gentamycin or trimethoprim. Although it was not possible to identify antibiotic resistant bacteria of the genus Vibrio, resistant strains of Aeromonas hydrophila with a wide variety of resistance patterns could be cultured in many cases. R plasmids were found by means of conjugation in 21.2% of the 826 tested Aeromonas hydrophila strains. They were characterized with the aid of the fin- and pili test. The majority of the R plasmids were fin-negative, and the sex pili could be assigned strikingly often to sex pilus group C with the phages we used. 15 strains of Aeromonas hydrophila had been tested in agarose gel electrophoresis on plasmid DNA. In 11 Aeromonas hydrophila strains with transferable and in 3 from 4 strains with non transferable multiple antibiotic resistance plasmid DNA with 100 Megadalton had been found.
已对262份河水样本进行分析，以检测抗生素抗性气单胞菌属和弧菌属菌株的存在情况。这些细菌在含有抗菌化疗药物土霉素、氯霉素、链霉素、氨苄青霉素、卡那霉素、庆大霉素或甲氧苄啶的选择性培养基上培养。虽然无法鉴定出弧菌属的抗生素抗性细菌，但在许多情况下，可以培养出具有多种抗性模式的嗜水气单胞菌抗性菌株。在826株受试嗜水气单胞菌菌株中，有21.2%通过接合作用发现了R质粒。借助于菌毛和性菌毛试验对其进行了鉴定。大多数R质粒是菌毛阴性的，而且使用我们的噬菌体时，性菌毛常常可以显著地归为C型性菌毛组。对15株嗜水气单胞菌进行了质粒DNA的琼脂糖凝胶电泳检测。在11株具有可转移多重抗生素抗性的嗜水气单胞菌菌株以及4株非可转移多重抗生素抗性的嗜水气单胞菌菌株中的3株中，发现了100兆道尔顿的质粒DNA。
