Rüther L, Bolke L, Schlippe G R, Voss W A
, Engelstr. 37, 48143, Münster, Deutschland.
Hautarzt. 2017 Dec;68(12):987-993. doi: 10.1007/s00105-017-4054-9.
Wound healing can be divided into three phases: (1) exsudation phase, (2) granulation phase, (3) regeneration phase. In particular, the epithelization phase is of great importance in order to quickly reconstitute the natural skin barrier. The aim of the present study was to determine the reepithelization kinetics of untreated and 0.5% sodium hyaluronate (NHA) treated human 3D full thickness skin models.
The test protocol consisted of topically applying 10 µl of the test substance 0.5% NHA twice a day. Evaluation of reepithelialization kinetics was carried out from days 2-6. Determination of the influence on immune response was performed based on quantification of IL-1α and IL-10.
Application of 0.5% NHA twice a day enhanced the reepithelialization speed at all time points (p < 0.001). This observation is accompanied by a reduced expression of IL-10 paralleled by an elevated expression of IL-1α on days 2-4 (p < 0.001).
The treatment of human skin models with NHA resulted in a significantly increased reepithelization velocity of wounded tissue and consequently promoted faster wound closure, compared to untreated controls. It can be assumed that the downregulation of IL-10 caused the IL1-α mediated increased immune response which finally leads to accelerated wound healing. Follow-up studies will reveal if the faster wound healing and the modulation of the immune response through the application of NHA is valid in vivo.
伤口愈合可分为三个阶段：（1）渗出期，（2）肉芽期，（3）再生期。特别是上皮形成期对于快速重建天然皮肤屏障非常重要。本研究的目的是确定未处理和经0.5%透明质酸钠（NHA）处理的人3D全层皮肤模型的再上皮化动力学。
试验方案包括每天两次局部涂抹10μl 0.5%的NHA受试物质。在第2至6天对再上皮化动力学进行评估。基于IL-1α和IL-10的定量测定对免疫反应的影响。
每天两次涂抹0.5%的NHA在所有时间点均提高了再上皮化速度（p < 0.001）。这一观察结果伴随着IL-10表达的降低，同时在第2至4天IL-1α表达升高（p < 0.001）。
与未处理的对照相比，用NHA处理人皮肤模型导致受伤组织的再上皮化速度显著增加，从而促进伤口更快愈合。可以假设，IL-10的下调导致IL1-α介导的免疫反应增加，最终导致伤口愈合加速。后续研究将揭示通过应用NHA实现的更快伤口愈合和免疫反应调节在体内是否有效。
