Wu Song, Deng Liang, Hsia Hanson, Xu Kai, He Yu, Huang Qiangru, Peng Yi, Zhou Zhihua, Peng Cheng
1 Department of orthopedics of the 3rd Xiangya Hospital, Central South University, Changsha, China.
2 Xiangya medical school, Central South University, Changsha, China.
J Biomater Appl. 2017 May;31(10):1380-1390. doi: 10.1177/0885328217702526. Epub 2017 Apr 4.
Excellent wound dressings maintain a warm and moist environment, thus accelerating wound healing. In this study, we cross-linked gelatin and hyaluronic acid with ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride in different ratios (gelatin/hyaluronic acid = 8:2, gelatin/hyaluronic acid = 5:5, gelatin/hyaluronic acid = 2:8), and explored the effects and mechanisms of gelatinhyaluronic acid hydrogels on wound healing. This was done by examining dressing properties, such as fluid uptake ability, water vapor transmission rate, and the rate of water evaporation. We further verified biological function by using in vitro and in vivo wound models. The hydrogels display appropriate fluid uptake ability and good water vapor transmission rate and rate of water evaporation all of which can provide an adequate moisture environment for wound healing. Cell cytotoxicity and proliferation tests show that the hydrogels have no cytotoxicity, furthermore, gelatin/hyaluronic acid = 8:2 hydrogels have the potential to promote cell proliferation. Animal wound models demonstrate that the hydrogels can effectively promote wound healing in vivo, in particular, the gelatin/hyaluronic acid = 8:2 group which promoted the most rapid healing. Accordingly, gelatin-hyaluronic acid dressings, especially the gelatin/hyaluronic acid = 8:2 hydrogels, have a promising outlook for clinical applications in wound healing.
优质的伤口敷料能保持温暖湿润的环境，从而加速伤口愈合。在本研究中，我们将明胶和透明质酸与盐酸N-（3-二甲氨基丙基）-N'-乙基碳二亚胺以不同比例交联（明胶/透明质酸 = 8:2、明胶/透明质酸 = 5:5、明胶/透明质酸 = 2:8），并探究了明胶-透明质酸水凝胶对伤口愈合的影响及机制。通过检测敷料性能，如吸液能力、水蒸气透过率和水分蒸发速率来实现。我们还通过体外和体内伤口模型进一步验证了其生物学功能。这些水凝胶表现出合适的吸液能力、良好的水蒸气透过率和水分蒸发速率，所有这些都能为伤口愈合提供适宜的湿润环境。细胞毒性和增殖试验表明，这些水凝胶无细胞毒性，此外，明胶/透明质酸 = 8:2的水凝胶有促进细胞增殖的潜力。动物伤口模型表明，这些水凝胶能在体内有效促进伤口愈合，尤其是明胶/透明质酸 = 8:2组，其促进愈合的速度最快。因此，明胶-透明质酸敷料，尤其是明胶/透明质酸 = 8:2的水凝胶，在伤口愈合的临床应用方面前景广阔。
