急性给予乙醇对大鼠肝脏谷胱甘肽代谢的影响。

Influence of acute ethanol administration on hepatic glutathione metabolism in the rat.

作者信息

Gonzalez J, Muñoz M E, Martin M I, Collado P S, Fermoso J, Esteller A

机构信息

Department of Physiology and Pharmacology, University of Salamanca, Spain.

出版信息

Alcohol. 1988 Mar-Apr;5(2):103-6. doi: 10.1016/0741-8329(88)90004-3.

DOI:10.1016/0741-8329(88)90004-3

PMID:2899436

Abstract

The effect of acute ethanol administration on the hepatic metabolism of glutathione was studied in male Wistar rats. Animals fasted for 18 hr received ethanol (5 g/kg body wt.) through a gastric tube as a 20% (w/v) solution in 0.154 NaCl. Four hours after administration of ethanol liver glutathione content was decreased by 21% when compared to saline-treated controls. A significant reduction (28%) was also found in gamma-glutamylcysteine synthetase activity and plasma glutathione levels were increased non significantly by 17% with respect to control rats. Glutathione S-transferase activity in the liver of ethanol-treated animals was decreased by 28% but no change was found in total glutathione peroxidase activity. The results indicate that the lowered glutathione synthesis could be an important factor contributing to the reduction of hepatic glutathione concentration following the acute ingestion of ethanol.

摘要

在雄性Wistar大鼠中研究了急性给予乙醇对肝脏谷胱甘肽代谢的影响。禁食18小时的动物通过胃管接受乙醇（5克/千克体重），以20%（w/v）的溶液形式溶于0.154 NaCl中。给予乙醇4小时后，与用生理盐水处理的对照组相比，肝脏谷胱甘肽含量降低了21%。γ-谷氨酰半胱氨酸合成酶活性也显著降低（28%），并且与对照大鼠相比，血浆谷胱甘肽水平无显著升高，升高了17%。乙醇处理动物肝脏中的谷胱甘肽S-转移酶活性降低了28%，但总谷胱甘肽过氧化物酶活性未发现变化。结果表明，谷胱甘肽合成降低可能是急性摄入乙醇后导致肝脏谷胱甘肽浓度降低的一个重要因素。