通过微流控管进行结晶可实现原位和异位X射线衍射。
Crystallization via tubing microfluidics permits both in situ and ex situ X-ray diffraction.
作者信息
Gerard Charline J J, Ferry Gilles, Vuillard Laurent M, Boutin Jean A, Chavas Leonard M G, Huet Tiphaine, Ferte Nathalie, Grossier Romain, Candoni Nadine, Veesler Stéphane
机构信息
CINaM-CNRS, Aix-Marseille Université, Campus de Luminy, Case 913, 13288 Marseille CEDEX 09, France.
Institut de Recherches Servier, 125 Chemin de Ronde, 78290 Croissy-sur-Seine, France.
出版信息
Acta Crystallogr F Struct Biol Commun. 2017 Oct 1;73(Pt 10):574-578. doi: 10.1107/S2053230X17013826. Epub 2017 Oct 2.
Abstract
A microfluidic platform was used to address the problems of obtaining diffraction-quality crystals and crystal handling during transfer to the X-ray diffractometer. Crystallization conditions of a protein of pharmaceutical interest were optimized and X-ray data were collected both in situ and ex situ.
摘要
一个微流控平台被用于解决在将晶体转移至X射线衍射仪的过程中获得衍射质量晶体以及晶体处理的问题。对一种具有药物研究价值的蛋白质的结晶条件进行了优化，并原位和非原位收集了X射线数据。
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