Gaibani Paolo, Scaltriti Erika, Benni Chiara, Pongolini Stefano, Ambretti Simone, Landini Maria Paola, Viale PierLuigi, Giannella Maddalena, Re Maria Carla
Operative Unit of Clinical Microbiology, St. Orsola-Malpighi University Hospital, Bologna, Italy.
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), Direzione Sanitaria-Servizio di Analisi del Rischio, Parma, Italy.
New Microbiol. 2017 Oct;40(4):284-285. Epub 2017 Oct 10.
Here we report the complete nucleotide sequence of a 49.257-bp IncL/M conjugative plasmid (pRAY) carrying the blaOXA-48 gene collected from a Klebsiella pneumoniae clinical strain isolated in Italy. The genetic environment of pRAY plasmid revealed that the blaOXA-48 gene was located within a Tn1999.2 transposon. The pRAY plasmid differed from blaOXA-48-harboring IncL/M plasmids by genetic context and size. Comparative analysis demonstrated that pRAY plasmid lacked a region of ~15 kb carrying genes encoding proteins involved in pilus assembly and plasmid conjugative apparatus.
在此，我们报告了从意大利分离的一株肺炎克雷伯菌临床菌株中收集到的携带blaOXA - 48基因的49257 bp IncL/M接合质粒（pRAY）的完整核苷酸序列。pRAY质粒的遗传环境显示，blaOXA - 48基因位于Tn1999.2转座子内。pRAY质粒在遗传背景和大小上与携带blaOXA - 48的IncL/M质粒不同。比较分析表明，pRAY质粒缺少一个约15 kb的区域，该区域携带编码参与菌毛组装和质粒接合装置的蛋白质的基因。
