He Ran, Wang Hong-Jin, Zhou Ying, Li Jing-Jing, Xu Xiao-Yu
Chongqing Engineering Research Center for Pharmacodynamics Evaluation, College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing 400715, China.
Zhongguo Zhong Yao Za Zhi. 2017 Mar;42(5):944-950. doi: 10.19540/j.cnki.cjcmm.20170121.012.
To investigate the effect of Siwu decoction on improving iron deficiency anemia in infant rats and observe its regulatory effects on iron metabolism. SD rats were fed with low iron fodder for 2 weeks, and then the rats with hemoglobin level less than 75 g•L ⁻¹ were screened out and randomly divided into model group, Ferrous succinate 50 mg•kg ⁻¹ group, Siwu decoction 4 g•kg ⁻¹, 8 g•kg ⁻¹ and 16 g•kg ⁻¹ groups. After 4 weeks' gavage administration, Wright-Giemsa's staining of blood smear and HE staining of the livers were conducted, and all rats were tested for blood routine, serum iron, total iron binding capacity, serum ferritin, serum hepcidin and liver hepcidin. The expression levels of liver ferritin, transferrin and transferrin receptor 1 were also detected. The results showed that as compared with normal group, the activity level of model group was decreased, and the color and lustre of auricles and toes were pale white; the number of red blood cells was decreased; the volume was smaller, with an increased zone of central pallor; the body weight and blood routine parameters were decreased significantly; the livers were pale red, and the hepatic cords around thecentral veins were unclear and misaligned; the serum iron, serum ferritin, liver iron levels and the expression of liver ferritin were decreased significantly; the total iron binding capacity, serum hepcidin, liver hepcidin, the expression levels of liver transferrin and transferrin receptor 1 were significantly increased, indicating successful establishment of models. As compared with the model group, activity was increased in Siwu decoction group; the color and lustre of auricles and toes were ruddy; the number of red blood cells was increased; the volume was larger, with a decreased zone of central pallor; the body weight and blood routine parameters were increased significantly; the livers were red, hepatic cords around the central veins were clear and aligned;the serum iron, serum ferritin, liver iron levels and the expression of liver ferritin were significantly increased, the total iron binding capacity, serum hepcidin, liver hepcidin, the expression of liver transferrin and transferrin receptor 1 were decreased significantly. The results demonstrated that Siwu decoction had a certain effect on improving iron deficiency anemia in infant rats, and the mechanism may be associated with the regulatory effect of hepcidin iron metabolism.
探讨四物汤对改善幼龄大鼠缺铁性贫血的作用，并观察其对铁代谢的调节作用。将SD大鼠喂饲低铁饲料2周，然后筛选出血红蛋白水平低于75 g•L⁻¹的大鼠，随机分为模型组、琥珀酸亚铁50 mg•kg⁻¹组、四物汤4 g•kg⁻¹组、8 g•kg⁻¹组和16 g•kg⁻¹组。灌胃给药4周后，进行血涂片的瑞氏-吉姆萨染色和肝脏的HE染色，并检测所有大鼠的血常规、血清铁、总铁结合力、血清铁蛋白、血清铁调素和肝脏铁调素。同时检测肝脏铁蛋白、转铁蛋白和转铁蛋白受体1的表达水平。结果显示，与正常组相比，模型组活动水平降低，耳廓和脚趾色泽苍白；红细胞数量减少；体积变小，中央淡染区增大；体重和血常规参数显著降低；肝脏呈淡红色，中央静脉周围肝索不清且排列紊乱；血清铁、血清铁蛋白、肝脏铁水平及肝脏铁蛋白表达显著降低；总铁结合力、血清铁调素、肝脏铁调素、肝脏转铁蛋白和转铁蛋白受体１表达水平显著升高，表明模型建立成功。与模型组相比，四物汤组活动增加；耳廓和脚趾色泽红润；红细胞数量增加；体积变大，中央淡染区减小；体重和血常规参数显著增加；肝脏呈红色，中央静脉周围肝索清晰且排列整齐；血清铁、血清铁蛋白、肝脏铁水平及肝脏铁蛋白表达显著增加，总铁结合力、血清铁调素、肝脏铁调素、肝脏转铁蛋白和转铁蛋白受体1表达显著降低。结果表明，四物汤对改善幼龄大鼠缺铁性贫血有一定作用，其机制可能与铁调素对铁代谢的调节作用有关。
