Wang Yu, Lin Zhi-Jian, Nie An-Zheng, Li Li-Yu, Zhang Bing
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.
Zhongguo Zhong Yao Za Zhi. 2017 Mar;42(5):958-963. doi: 10.19540/j.cnki.cjcmm.2017.0029.
Sixty SD male rats were randomly divided into normal group, model group, benzbromarone group(20 mg•kg⁻¹•d⁻¹), chicory extract high dose, middle dose and low dose groups (5, 7.5, 10 g•kg⁻¹•d⁻¹). The rats in normal group were given with water, and the rats in other groups were given with 10% fructose solution to establish hyperuricemia models. All the rats were sacrificed on the 42th day. Then their serum uric acid(SUA), serum creatinine(CRE), urea nitrogen(BUN) and urinary uric acid(UUA) levels were detected to calculate the clearance rate of uric acid in kidney(CUA). Meanwhile, the protein and gene expression levels of renal glucose transporter family member 9(Glut9) were detected by immunohistochemical and Real-time quantitative reverse transcription-polymerase chain reaction(RT-qPCR) methods. The effects of Chinese herb chicory extract on expression of renal Glut9 and decreasing uric acid were explored in this study, and the results showed that chicory extract could reduce SUA level in rats with hyperuricemia, increase renal CUA, decrease the protein expression of renal Glut9, inhibit uric acid re-absorption in kidney, and thus promote renal uric acid excretion.
将60只雄性SD大鼠随机分为正常组、模型组、苯溴马隆组（20毫克•千克⁻¹•天⁻¹）、菊苣提取物高剂量组、中剂量组和低剂量组（5、7.5、10克•千克⁻¹•天⁻¹）。正常组大鼠给予水，其他组大鼠给予10%果糖溶液以建立高尿酸血症模型。所有大鼠在第42天处死。然后检测其血清尿酸（SUA）、血清肌酐（CRE）、尿素氮（BUN）和尿尿酸（UUA）水平，计算肾脏尿酸清除率（CUA）。同时，采用免疫组织化学和实时定量逆转录-聚合酶链反应（RT-qPCR）方法检测肾葡萄糖转运蛋白家族成员9（Glut9）的蛋白和基因表达水平。本研究探讨了中药菊苣提取物对肾Glut9表达及降尿酸的影响，结果表明菊苣提取物可降低高尿酸血症大鼠的SUA水平，提高肾脏CUA，降低肾Glut9的蛋白表达，抑制肾脏尿酸重吸收，从而促进肾脏尿酸排泄。
