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钛盘表面粗糙度及褪黑素局部应用对成骨细胞反应的体外初步研究

In vitro preliminary study of osteoblast response to surface roughness of titanium discs and topical application of melatonin.

作者信息

Solá-Ruiz Maria-Fernanda, Pérez-Martínez Carolina, Martín-del-Llano José-Javier, Carda-Batalla Carmen, Labaig-Rueda Carlos

机构信息

Clínica Odontológica, C/ Gascó Oliag 1, 46010 Valencia, Spain,

出版信息

Med Oral Patol Oral Cir Bucal. 2015 Jan 1;20(1):e88-93. doi: 10.4317/medoral.19953.

DOI:10.4317/medoral.19953
PMID:25129252
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4320427/
Abstract

OBJECTIVES

To observe human osteoblast behavior cultured in vitro on titanium discs (Ti) in relation to surface roughness and melatonin application.

STUDY DESIGN

Human osteoblasts (MG-63) were cultured on 60 Ti6Al4V discs divided into three groups: Group I: discs treated with dual acid etching; Group II dual acid etching and blasting with calcium phosphate particles; Group III (control) machined discs. Surface roughness and topography of the discs were examined with scanning electron microscope (SEM) and confocal laser scanning electron microscope( CLSM). Osteoblast adhesion, proliferation and cell morphology were determined by means of fluorescence microscopy with Image-Pro Plus software and SEM.

RESULTS

Group II presented the roughest discs, while the least rough were Group III. Cell adhesion was greatest in Group II. The addition of melatonin improved cell proliferation.

CONCLUSIONS

  1. Surface treatments (dual acid etching, calcium phosphate impaction) increase surface roughness in comparison with machined titanium. 2. Greater surface roughness tends to favor cell adhesion after 24-hour cell culture. 3. The addition of melatonin tends to favor osteoblast proliferation.
摘要

目的

观察体外培养于钛盘（Ti）上的人成骨细胞行为与表面粗糙度及褪黑素应用之间的关系。

研究设计

将人成骨细胞（MG - 63）培养在60个Ti6Al4V钛盘上，分为三组：第一组：经双酸蚀刻处理的钛盘；第二组：双酸蚀刻并使用磷酸钙颗粒喷砂处理的钛盘；第三组（对照组）：机械加工的钛盘。用扫描电子显微镜（SEM）和共聚焦激光扫描电子显微镜（CLSM）检查钛盘的表面粗糙度和形貌。通过带有Image - Pro Plus软件的荧光显微镜和SEM测定成骨细胞的黏附、增殖及细胞形态。

结果

第二组的钛盘表面最粗糙，而第三组最光滑。第二组成骨细胞黏附能力最强。添加褪黑素可促进细胞增殖。

结论

  1. 与机械加工的钛相比，表面处理（双酸蚀刻、磷酸钙撞击）可增加表面粗糙度。2. 更大的表面粗糙度在细胞培养24小时后往往有利于细胞黏附。3. 添加褪黑素往往有利于成骨细胞增殖。
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f730/4320427/d30c0a195ec6/medoral-20-e88-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f730/4320427/66c596648159/medoral-20-e88-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f730/4320427/671a0529a035/medoral-20-e88-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f730/4320427/d30c0a195ec6/medoral-20-e88-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f730/4320427/66c596648159/medoral-20-e88-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f730/4320427/671a0529a035/medoral-20-e88-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f730/4320427/d30c0a195ec6/medoral-20-e88-g003.jpg

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