Rodriguez M N, Gomez-Pan A, Arilla E
Departamento de Bioquimica y Biologia Molecular, Facultad de Medicina, Universidad de Alcala de Henares, Madrid, Spain.
Endocrinology. 1988 Aug;123(2):1147-52. doi: 10.1210/endo-123-2-1147.
The effects of adrenalectomy on somatostatin (SS) concentration and specific binding in cerebral cortex, hippocampus, striatum, and hypothalamus were examined using [125I-Tyr11]SS as the binding ligand. Adrenalectomy did not affect the concentration of SS-like immunoreactivity in the brain areas studied. Nevertheless, the number of SS receptors was significantly decreased in membrane preparations from hippocampus, striatum, and hypothalamus, but not in cerebral cortex. No significant differences in the apparent binding affinity values were seen after adrenalectomy. The adrenalectomy-induced decreases in [125I-Tyr11]SS receptors were completely reversed by glucocorticoid replacement with dexamethasone. These results demonstrate that adrenalectomy modulates SS receptors in discrete brain areas, suggesting the existence of a possible relationship between corticosteroids and SS in the neuronal activity of these structures. The physiological significance of these findings remains to be clarified.
使用[125I-酪氨酸11]生长抑素（SS）作为结合配体，研究了肾上腺切除术对大脑皮质、海马体、纹状体和下丘脑生长抑素（SS）浓度及特异性结合的影响。肾上腺切除术并未影响所研究脑区中SS样免疫反应性的浓度。然而，海马体、纹状体和下丘脑膜制剂中SS受体的数量显著减少，但大脑皮质中未减少。肾上腺切除术后，表观结合亲和力值未见显著差异。用 dexamethasone进行糖皮质激素替代可完全逆转肾上腺切除术诱导的[125I-酪氨酸11]SS受体减少。这些结果表明，肾上腺切除术可调节特定脑区的SS受体，提示在这些结构的神经元活动中，皮质类固醇与SS之间可能存在某种关系。这些发现的生理学意义仍有待阐明。
