一种筛选缺乏D-氨基酸氧化酶活性的突变小鼠的简单快速方法。

A simple and rapid method to screen for mutant mice lacking D-amino-acid oxidase activity.

作者信息

Konno R, Yasumura Y

机构信息

Department of Microbiology, Dokkyo University School of Medicine, Tochigi, Japan.

出版信息

Lab Anim Sci. 1988 Jun;38(3):292-5.

PMID:2900911

Abstract

A simple and rapid method to screen for mutant mice (Mus musculus) lacking D-amino-acid oxidase activity has been devised. Mice were given water containing small amounts (0.02%) of either D-methionine or D-phenylalanine. Urinary levels of the D-amino acid were examined using thin-layer chromatography. Some mice excreted substantial amounts of the D-amino acid through the urine. None of them had detectable D-amino-acid oxidase activity.

摘要

已设计出一种简单快速的方法来筛选缺乏D-氨基酸氧化酶活性的突变小鼠（小家鼠）。给小鼠饮用含有少量（0.02%）D-蛋氨酸或D-苯丙氨酸的水。使用薄层色谱法检测尿液中D-氨基酸的水平。一些小鼠通过尿液排出大量的D-氨基酸。它们均未检测到D-氨基酸氧化酶活性。