Nantulya V M, Moloo S K
International Laboratory for Research on Animal Diseases (ILRAD), Nairobi, Kenya.
Acta Trop. 1988 Jun;45(2):137-44.
Five hundred and sixty teneral male Glossina morsitans centralis were fed, at the height of parasitaemia, on a goat infected with Trypanosoma brucei brucei. Thereafter, the tsetse were divided into 4 equal groups. Group I was fed in vitro once weekly for 4 weeks and Group II twice weekly for 4 weeks on fresh defibrinated ox blood containing 2 mg/ml purified monoclonal antibody against T. b. brucei procyclics, while Group III was fed twice a week for 4 weeks on blood containing 2 mg/ml anti-T. vivax monoclonal antibody. The last group was fed on a rabbit. The tsetse were dissected on day 31 and the percent salivary gland infection rates observed were 18.2, 18.6, 39.8 and 40.8, respectively. In another experiment, 2 groups of tsetse, 120 per group, were fed on fresh defibrinated ox blood containing 2 mg/ml anti-T. b. brucei (test group) or anti-T. vivax (control group), on days 3, 6 and 9 following the infected feed. Dissection of the tsetse on day 31 revealed salivary gland infection rates of 0% in the test group and 6.5% in the control group. Thus the monoclonal antibody had a marked, specific suppression of the cyclical development of T. b. brucei in the tsetse vector.
在寄生虫血症高峰期，用感染了布氏布氏锥虫的山羊对560只羽化后不久的雄性中喙采采蝇进行喂食。此后，将采采蝇分成4个相等的组。第一组每周在体外喂食含2毫克/毫升抗布氏布氏锥虫前循环体纯化单克隆抗体的新鲜去纤维蛋白牛血，共4周；第二组每周喂食两次，共4周；第三组每周喂食含2毫克/毫升抗间日疟原虫单克隆抗体的血液，共4周。最后一组用兔子喂食。在第31天解剖采采蝇，观察到的唾液腺感染率分别为18.2%、18.6%、39.8%和40.8%。在另一项实验中，两组采采蝇，每组120只，在感染性喂食后的第3、6和9天，用含2毫克/毫升抗布氏布氏锥虫（试验组）或抗间日疟原虫（对照组）的新鲜去纤维蛋白牛血喂食。在第31天解剖采采蝇，试验组唾液腺感染率为0%，对照组为6.5%。因此，单克隆抗体对采采蝇载体中布氏布氏锥虫的循环发育有显著的特异性抑制作用。
