De la Rosa-Ramos Miguel A, Muñoz-Solís Karina, Palma-Zepeda Mario, Gutierrez-Castillo Adriana C, López Villegas Edgar O, Guerra-Infante Fernando M, Castro-Escarpulli Graciela
a Laboratorio de Microbiología Veterinaria, Departamento de Microbiología de la Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional , Mexico City , Mexico.
b Laboratorio de Bacteriología Médica, Departamento de Microbiología de la Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional , Mexico City , Mexico.
Avian Pathol. 2018 Apr;47(2):172-178. doi: 10.1080/03079457.2017.1390208. Epub 2017 Oct 30.
Ornithobacterium rhinotracheale is a bacterium that causes respiratory disease in birds and it has been isolated in countries with a large poultry production, including Mexico. The pathogenicity mechanisms of this bacterium have not been completely elucidated yet. The capacity of the bacterium to adhere to epithelial cells of chicken in vitro has been evidenced, and since this bacterium has been isolated from the lungs and air sacs of several avian species, the aim of this study was to determine if this bacterium can adhere to chicken lung cells. We used five O. rhinotracheale reference serovars (A-E) that were in contact with primary lung cells cultured from a 19-day-old chicken embryo. O. rhinotracheale adherence was evaluated through optical and transmission electron microscopies. The results revealed that O. rhinotracheale is capable of adhering to chicken embryo lung cells within 3 h of incubation with a diffuse adherence pattern. The adherence percentages of the chicken embryo lung cells were 51-96% according to the serovar of the bacterium. Relative adherence was from 4 to 8 bacteria per cell. Transmission electron microscope data revealed intracellular bacteria inside a vacuole in less than 3 h of incubation.
禽气管鸟杆菌是一种可导致禽类呼吸道疾病的细菌，在包括墨西哥在内的家禽养殖大国均有分离出该细菌。这种细菌的致病机制尚未完全阐明。已证实该细菌在体外具有黏附鸡上皮细胞的能力，并且由于该细菌已从多种禽类的肺和气囊中分离出来，本研究的目的是确定这种细菌是否能黏附鸡肺细胞。我们使用了五个禽气管鸟杆菌参考血清型（A - E），使其与从19日龄鸡胚培养的原代肺细胞接触。通过光学显微镜和透射电子显微镜评估禽气管鸟杆菌的黏附情况。结果显示，禽气管鸟杆菌在孵育3小时内能够以弥漫性黏附模式黏附鸡胚肺细胞。根据细菌的血清型，鸡胚肺细胞的黏附率为51% - 96%。相对黏附数为每个细胞有4至8个细菌。透射电子显微镜数据显示，在孵育不到3小时的时间内，液泡内存在细胞内细菌。
