Chaudhary Raskin, Thapa Shrawan Kumar, Rana Jid Chani, Shah Pradeep Kumar
Department of Microbiology, St. Xavier's College, Kathmandu, Nepal.
Orthopaedic Department, Bharatpur Hospital, Chitwan, Nepal.
J Nepal Health Res Counc. 2017 Sep 8;15(2):120-123. doi: 10.3126/jnhrc.v15i2.18185.
Post-operative surgical site infections (SSIs) are among the leading cause of morbidity and increased medical expense. The aim of this study is to isolate identify and study antimicrobial susceptibility pattern of microorganism from surgical wound of admitted patients.
This retrospective study was carried at the Microbiology Laboratory of Bharatpur hospital, Nepal, from May 2015 to October 2015. The pus samples were cultured and antibiotic susceptibility determined in vitro by Kirby Bauer's disc diffusion method following clinical and Laboratory Standards Institute (CLSI) 2014 recommendation.
Of the total 250 samples, 194 (77.6%) showed bacterial growth. Staphylococcus aureus was 47.4% and Escherichia coli 20.60 %. Of 194 isolates 39.2% were multi drug resistant. Amikacin was sensitive in 93.1% of Gram positive isolates and 81.8% of gram negative isolates.
Bacterial growth is common in surgical site. Staphylococcus aureus and Escherichia coli were multidrug resistant. Grampositive and gram negative isolates were commonly sensitive to Amikacin.
术后手术部位感染（SSIs）是发病率和医疗费用增加的主要原因之一。本研究的目的是从入院患者的手术伤口中分离、鉴定并研究微生物的抗菌药敏模式。
本回顾性研究于2015年5月至2015年10月在尼泊尔巴拉特普尔医院微生物实验室进行。按照临床和实验室标准协会（CLSI）2014年的建议，采用 Kirby Bauer 纸片扩散法对脓液样本进行体外培养和抗生素敏感性测定。
在总共250个样本中，194个（77.6%）显示有细菌生长。金黄色葡萄球菌占47.4%，大肠杆菌占20.60%。在194株分离菌中，39.2%为多重耐药菌。阿米卡星对93.1%的革兰氏阳性分离菌和81.8%的革兰氏阴性分离菌敏感。
手术部位细菌生长常见。金黄色葡萄球菌和大肠杆菌具有多重耐药性。革兰氏阳性和革兰氏阴性分离菌通常对阿米卡星敏感。
