Miyan Zahid, Fawwad Asher, Sabir Rubina, Basit Abdul
Baqai Institute of Diabetology and Endocrinology, Baqai Medical University, Karachi.
J Pak Med Assoc. 2017 May;67(5):665-669.
To determine the microbiological profile and antibiotic susceptibility patterns of organisms isolated from diabetic foot ulcers in a tertiary care hospital Karachi, Pakistan.
This descriptive study was conducted at the Baqai Institute of Diabetology and Endocrinology (BIDE), from January 2013 to March 2014. Bone, pus and tissue samples were collected from 342 patients with diabetic foot infections and inoculated on appropriate media. Antibiotic susceptibility tests were done by Kirby Bauer disk diffusion method.
A total of 671 aerobic bacteria were isolated from 473 specimens with an average of 1.45 isolates per specimen. Poly-microbial infection was 56.87%. Gram- negative isolates were predominant 76.27%.Staphylococcus aureus was most frequent among Gram-positive 20.7% and Escherichia coli 15.72% in Gram negative isolates. MRSA was found in 26.76% Staphylococcus aureus. About 33.48% of antimicrobial resistant isolates were observed.
In our study, Gram negative aerobes were predominant in the diabetic foot infections. A significant number of MDR isolates were also observed in this cohort. Delayed referral and inappropriate use of broad spectrum antibiotics may be the main cause of increase in the frequency of MDR isolates.
确定从巴基斯坦卡拉奇一家三级护理医院的糖尿病足溃疡中分离出的微生物谱及抗生素敏感性模式。
本描述性研究于2013年1月至2014年3月在巴基糖尿病与内分泌研究所（BIDE）进行。从342例糖尿病足感染患者中采集骨、脓液和组织样本，并接种于合适的培养基上。采用 Kirby Bauer 纸片扩散法进行抗生素敏感性试验。
从473份标本中总共分离出671株需氧菌，每份标本平均分离出1.45株。混合感染率为56.87%。革兰氏阴性菌分离株占主导，为76.27%。革兰氏阳性菌中金黄色葡萄球菌最为常见，占20.7%，革兰氏阴性菌中大肠杆菌占15.72%。耐甲氧西林金黄色葡萄球菌（MRSA）在金黄色葡萄球菌中占26.76%。观察到约33.48%的抗菌药物耐药分离株。
在我们的研究中，革兰氏阴性需氧菌在糖尿病足感染中占主导。在该队列中也观察到大量多重耐药分离株。延迟转诊和广谱抗生素的不当使用可能是多重耐药分离株频率增加的主要原因。
