聚合物纳米粒子与血清蛋白的纳-生物界面上的浓度依赖性蛋白质吸附。
Concentration-dependent protein adsorption at the nano-bio interfaces of polymeric nanoparticles and serum proteins.
机构信息
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
出版信息
Nanomedicine (Lond). 2017 Nov;12(22):2757-2769. doi: 10.2217/nnm-2017-0238. Epub 2017 Oct 10.
AIM
A comprehensive understanding of nanoparticle (NP)-protein interaction (protein corona formation) is required. So far, many factors influencing this interaction have been investigated, like size and ζ potential. However, NPs exposure concentration has always been ignored. Herein, we aim to disclose the correlation of NPs exposure concentration with protein adsorption.
MATERIALS & METHODS: Four polymeric NPs systems possessing similar sizes (230 ± 20 nm) but varied ζ potentials (-30 ∼ +40 mv) were prepared. Physicochemical properties and protein adsorption upon NP-protein interaction were characterized.
RESULTS
Protein adsorption capacity and adsorbed protein types were NPs concentration-dependent.
CONCLUSION
Considering the critical impacts of protein adsorption on NPs delivery, our work could be an urgent warning about the possible risks of dosage adjustment of nanoformulations.
目的
需要全面了解纳米颗粒（NP）-蛋白质相互作用（蛋白质冠形成）。到目前为止，已经研究了许多影响这种相互作用的因素，如大小和 ζ 电位。然而，一直忽略了 NPs 暴露浓度。在此，我们旨在揭示 NPs 暴露浓度与蛋白质吸附的相关性。
材料与方法
制备了四个具有相似尺寸（230±20nm）但 ζ 电位（-30∼+40mV）不同的聚合物 NP 系统。对 NP-蛋白质相互作用时的物理化学性质和蛋白质吸附进行了表征。
结果
蛋白质吸附量和吸附的蛋白质类型与 NPs 浓度有关。
结论
考虑到蛋白质吸附对 NPs 传递的关键影响，我们的工作可能对纳米制剂剂量调整的潜在风险发出了紧急警告。
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