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通过在镧系元素掺杂的上转换纳米颗粒上可控形成由变性牛血清白蛋白组成的蛋白质冠层来提高其胶体稳定性。

Controlled Formation of a Protein Corona Composed of Denatured BSA on Upconversion Nanoparticles Improves Their Colloidal Stability.

作者信息

Shanwar Samah, Liang Liuen, Nechaev Andrey V, Bausheva Daria K, Balalaeva Irina V, Vodeneev Vladimir A, Roy Indrajit, Zvyagin Andrei V, Guryev Evgenii L

机构信息

Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 603950 Nizhny Novgorod, Russia.

ARC Centre of Excellence "Nanoscale BioPhotonics", Department of Physics and Astronomy, Macquarie University, Sydney 2109, Australia.

出版信息

Materials (Basel). 2021 Mar 28;14(7):1657. doi: 10.3390/ma14071657.

DOI:10.3390/ma14071657
PMID:33800633
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8037850/
Abstract

In the natural fluidic environment of a biological system, nanoparticles swiftly adsorb plasma proteins on their surface forming a "protein corona", which profoundly and often adversely affects their residence in the systemic circulation in vivo and their interaction with cells in vitro. It has been recognized that preformation of a protein corona under controlled conditions ameliorates the protein corona effects, including colloidal stability in serum solutions. We report on the investigation of the stabilizing effects of a denatured bovine serum albumin (dBSA) protein corona formed on the surface of upconversion nanoparticles (UCNPs). UCNPs were chosen as a nanoparticle model due to their unique photoluminescent properties suitable for background-free biological imaging and sensing. UCNP surface was modified with nitrosonium tetrafluoroborate (NOBF) to render it hydrophilic. UCNP-NOBF nanoparticles were incubated in dBSA solution to form a dBSA corona followed up by lyophilization. As produced dBSA-UCNP-NOBF demonstrated high photoluminescence brightness, sustained colloidal stability after long-term storage and the reduced level of serum protein surface adsorption. These results show promise of dBSA-based nanoparticle pretreatment to improve the amiability to biological environments towards theranostic applications.

摘要

在生物系统的天然流体环境中，纳米颗粒会迅速在其表面吸附血浆蛋白，形成“蛋白质冠”，这会深刻且常常不利地影响它们在体内体循环中的留存以及它们在体外与细胞的相互作用。人们已经认识到，在可控条件下预先形成蛋白质冠可改善蛋白质冠效应，包括血清溶液中的胶体稳定性。我们报告了对上转换纳米颗粒（UCNPs）表面形成的变性牛血清白蛋白（dBSA）蛋白质冠的稳定作用的研究。由于UCNPs具有适用于无背景生物成像和传感的独特光致发光特性，因此被选作纳米颗粒模型。用四氟硼酸硝鎓（NOBF）对UCNP表面进行修饰，使其具有亲水性。将UCNP-NOBF纳米颗粒在dBSA溶液中孵育以形成dBSA冠，然后进行冻干。所制备的dBSA-UCNP-NOBF表现出高光致发光亮度、长期储存后持续的胶体稳定性以及血清蛋白表面吸附水平的降低。这些结果表明基于dBSA的纳米颗粒预处理有望改善其对生物环境的亲和性，以用于治疗诊断应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d42/8037850/425a10fa5608/materials-14-01657-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d42/8037850/b1a5aedaa7de/materials-14-01657-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d42/8037850/09d338514a3a/materials-14-01657-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d42/8037850/425a10fa5608/materials-14-01657-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d42/8037850/3eaaa7e2e277/materials-14-01657-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d42/8037850/7bdf9f639d73/materials-14-01657-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d42/8037850/948cd2c4e182/materials-14-01657-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d42/8037850/d94e84ce5f8c/materials-14-01657-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d42/8037850/f209411720a8/materials-14-01657-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d42/8037850/83edb487ca97/materials-14-01657-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d42/8037850/b1a5aedaa7de/materials-14-01657-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d42/8037850/09d338514a3a/materials-14-01657-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d42/8037850/425a10fa5608/materials-14-01657-g011.jpg

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