姜黄素是一种有前景的2型猪繁殖与呼吸综合征病毒感染抑制剂。
Curcumin is a promising inhibitor of genotype 2 porcine reproductive and respiratory syndrome virus infection.
作者信息
Du Taofeng, Shi Yunpeng, Xiao Shuqi, Li Na, Zhao Qin, Zhang Angke, Nan Yuchen, Mu Yang, Sun Yani, Wu Chunyan, Zhang Hongtao, Zhou En-Min
机构信息
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, Yangling, Shaanxi, 712100, China.
出版信息
BMC Vet Res. 2017 Oct 10;13(1):298. doi: 10.1186/s12917-017-1218-x.
BACKGROUND
Porcine reproductive and respiratory syndrome virus (PRRSV) could lead to pandemic diseases and huge financial losses to the swine industry worldwide. Curcumin, a natural compound, has been reported to serve as an entry inhibitor of hepatitis C virus, chikungunya virus and vesicular stomatitis virus. In this study, we investigated the potential effect of curcumin on early stages of PRRSV infection.
RESULTS
Curcumin inhibited infection of Marc-145 cells and porcine alveolar macrophages (PAMs) by four different genotype 2 PRRSV strains, but had no effect on the levels of major PRRSV receptor proteins on Marc-145 cells and PAMs or on PRRSV binding to Marc-145 cells. However, curcumin did block two steps of the PRRSV infection process: virus internalization and virus-mediated cell fusion.
CONCLUSIONS
Our results suggested that an inhibition of genotype 2 PRRSV infection by curcumin is virus strain-independent, and mainly inhibited by virus internalization and cell fusion mediated by virus. Collectively, these results demonstrate that curcumin holds promise as a new anti-PRRSV drug.
背景
猪繁殖与呼吸综合征病毒（PRRSV）可引发全球性疾病，给全球养猪业造成巨大经济损失。姜黄素是一种天然化合物，据报道可作为丙型肝炎病毒、基孔肯雅病毒和水疱性口炎病毒的进入抑制剂。在本研究中，我们调查了姜黄素对PRRSV感染早期阶段的潜在影响。
结果
姜黄素可抑制4种不同的2型PRRSV毒株对Marc-145细胞和猪肺泡巨噬细胞（PAM）的感染，但对Marc-145细胞和PAM上主要PRRSV受体蛋白的水平或PRRSV与Marc-145细胞的结合没有影响。然而，姜黄素确实阻断了PRRSV感染过程的两个步骤：病毒内化和病毒介导的细胞融合。
结论
我们的结果表明，姜黄素对2型PRRSV感染的抑制作用与病毒毒株无关，主要通过病毒内化和病毒介导的细胞融合受到抑制。总体而言，这些结果表明姜黄素有望成为一种新型抗PRRSV药物。
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