维甲酸诱导的大鼠肝脏转谷氨酰胺酶和总多胺在体内的调节作用。
Retinoic acid-induced modulation of rat liver transglutaminase and total polyamines in vivo.
作者信息
Piacentini M, Fesus L, Sartori C, Ceru M P
机构信息
Department of Biology, University of Rome Tor Vergata, Italy.
出版信息
Biochem J. 1988 Jul 1;253(1):33-8. doi: 10.1042/bj2530033.
The effect of a single intraperitoneal injection of retinoic acid on liver transglutaminase (EC 2.3.2.13) activity and total putrescine, spermidine and spermine was studied. The results demonstrate that: (1) transglutaminase activity is increased over control values as early as 4-6 h after treatment, reaching a maximum (2-fold increase) at 12 h and returning to control values at 36 h; (2) the retinoic acid-induced form of enzyme is the soluble tissue transglutaminase; (3) actinomycin D treatment does not completely inhibit the early (6 h) increase of activity, while suppressing that at 12 h; (4) the immunoassay of the soluble transglutaminase shows that, 6 h after treatment, there is no increase in the protein, whereas at 12 and 24 h a significant increase is observed; (5) putrescine, but not spermidine and spermine, increases (5-7-fold) 6 and 18 h after the retinoic acid treatment. The possibility also that the expression of soluble transglutaminase is modulated in vivo by retinoic acid and the relationship to polyamine levels are discussed.
研究了单次腹腔注射视黄酸对肝脏转谷氨酰胺酶（EC 2.3.2.13）活性以及总腐胺、亚精胺和精胺的影响。结果表明：（1）治疗后4 - 6小时，转谷氨酰胺酶活性即高于对照值，在12小时达到最大值（增加2倍），并在36小时恢复到对照值；（2）视黄酸诱导产生的酶形式为可溶性组织转谷氨酰胺酶；（3）放线菌素D处理不能完全抑制活性在早期（6小时）的增加，但能抑制12小时时的增加；（4）可溶性转谷氨酰胺酶的免疫分析表明，治疗后6小时，蛋白质没有增加，而在12小时和24小时观察到显著增加；（5）视黄酸处理后6小时和18小时，腐胺增加（5 - 7倍），而亚精胺和精胺没有增加。文中还讨论了视黄酸在体内调节可溶性转谷氨酰胺酶表达的可能性以及与多胺水平的关系。
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