Piacentini M, Sartori C, Beninati S, Bargagli A M, Cerù-Argento M P
Biochem J. 1986 Mar 1;234(2):435-40. doi: 10.1042/bj2340435.
Ornithine decarboxylase (ODC; EC 4.1.1.17), transglutaminase (EC 2.3.2.13), diamine oxidase (DAO; EC 1.4.3.6) and total di- and poly-amines were studied in rat liver and kidney cortex throughout pregnancy. In liver, ODC activity exhibited two major peaks (4.5-5 times the control activities) on days 15 and 17. Also putrescine and spermidine increased biphasically (3-4-fold), but no variation in spermine content was observed. Transglutaminase activity showed slight variations only near the end of gestation. In kidney, ODC activity did not fluctuate significantly during pregnancy, whereas both transglutaminase activity and putrescine content showed three major increases, in very early, middle and late pregnancy. No significant variations in spermidine and spermine were observed. In both organs, DAO activity, very low or undetectable until day 10, dramatically increased (10- and 20-fold in kidney and liver respectively) in the second half of pregnancy, reaching maxima on days 16-17 and 19. The results obtained for transglutaminase, ODC and total di- and poly-amines are interpreted on the basis of hyperplastic and hypertrophic events in the liver and kidney respectively. The behaviour of DAO suggests that the enzyme plays an important role in the control of intracellular diamine concentration.
在整个孕期对大鼠肝脏和肾皮质中的鸟氨酸脱羧酶（ODC；EC 4.1.1.17）、转谷氨酰胺酶（EC 2.3.2.13）、二胺氧化酶（DAO；EC 1.4.3.6）以及总二胺和多胺进行了研究。在肝脏中，ODC活性在第15天和第17天出现两个主要峰值（为对照活性的4.5 - 5倍）。腐胺和亚精胺也呈双相增加（3 - 4倍），但精胺含量未观察到变化。转谷氨酰胺酶活性仅在妊娠末期附近有轻微变化。在肾脏中，ODC活性在孕期没有显著波动，而转谷氨酰胺酶活性和腐胺含量在妊娠早期、中期和晚期均出现三次主要增加。亚精胺和精胺未观察到显著变化。在两个器官中，DAO活性在第10天之前非常低或无法检测到，在妊娠后半期急剧增加（肾脏和肝脏中分别增加10倍和20倍），在第16 - 17天和第19天达到最大值。根据肝脏和肾脏中分别发生的增生和肥大事件，对转谷氨酰胺酶、ODC以及总二胺和多胺的研究结果进行了解释。DAO的行为表明该酶在控制细胞内二胺浓度方面起重要作用。
