Blomhoff R, Holte K, Naess L, Berg T
Exp Cell Res. 1984 Jan;150(1):186-93. doi: 10.1016/0014-4827(84)90713-4.
We have recently shown that newly administered vitamin A (retinol) is initially taken up by the parenchymal cells of the liver, and subsequently (within 1-2 h) transferred to non-parenchymal liver cells (NPC) (Blomhoff et al., ref. [10]). In the present study we have separated the NPC by different methods to determine the cell type responsible for this uptake of [3H]retinol. When liver cells were prepared between 5 and 18 h after intraduodenal administration of [3H]retinol, the radioactive retinol was recovered mainly in the stellate cells. Other liver cells (i.e., hepatocytes, endothelial cells and Kupffer cells) contained only small amounts of [3H]retinol. Further, fluorescence microscopy studies indicated that stellate cells contain large quantities of retinol. Our results show that newly administered [3H]retinol, which is initially located in the hepatocytes, is transferred to the stellate cells and stored there.
我们最近发现，新给予的维生素A（视黄醇）最初被肝脏实质细胞摄取，随后（1-2小时内）转移至肝脏非实质细胞（NPC）（布洛姆霍夫等人，参考文献[10]）。在本研究中，我们通过不同方法分离了NPC，以确定负责摄取[3H]视黄醇的细胞类型。在十二指肠内给予[3H]视黄醇后5至18小时制备肝细胞时，放射性视黄醇主要在星状细胞中回收。其他肝细胞（即肝细胞、内皮细胞和库普弗细胞）仅含有少量[3H]视黄醇。此外，荧光显微镜研究表明星状细胞含有大量视黄醇。我们的结果表明，新给予的最初位于肝细胞中的[3H]视黄醇被转移至星状细胞并储存在那里。
