He Yanxia, Yuan Wangjun, Dong Meifang, Han Yuanji, Shang Fude
Plant Germplasm Resources and Genetic Laboratory, College of Life Sciences, Henan UniversityKaifeng, China.
Institute of Pharmacy, Pharmaceutical College of Henan UniversityKaifeng, China.
Front Plant Sci. 2017 Sep 22;8:1621. doi: 10.3389/fpls.2017.01621. eCollection 2017.
is an ornamental plant of substantial commercial value, and no genetic linkage maps of this species have previously been reported. Specific-locus amplified fragment sequencing (SLAF-seq) is a recently developed technology that allows massive single nucleotide polymorphisms (SNPs) to be identified and high-resolution genotyping. In our current research, we generated the first genetic map of using SLAF-seq, which is composed with 206.92 M paired-end reads and 173,537 SLAF markers. Among total 90,715 polymorphic SLAF markers, 15,317 polymorphic SLAFs could be used for genetic map construction. The integrated map contained 14,189 high quality SLAFs that were grouped in 23 genetic linkage groups, with a total length of 2962.46 cM and an average distance of 0.21 cM between two adjacent markers. In addition, 23,664 SNPs were identified from the mapped markers. As far as we know, this is the first of the genetic map of . Our results are further demonstrate that SLAF-seq is a very effective method for developing markers and constructing high-density linkage maps. The SNP markers and the genetic map reported in this study should be valuable resource in future research.
是一种具有重大商业价值的观赏植物，此前尚未有该物种的遗传连锁图谱报道。特定位点扩增片段测序（SLAF-seq）是一项最近开发的技术，它能够鉴定大量单核苷酸多态性（SNP）并进行高分辨率基因分型。在我们当前的研究中，我们利用SLAF-seq构建了第一张[物种名称未明确]的遗传图谱，该图谱由2.0692亿对末端测序 reads和173,537个SLAF标记组成。在总共90,715个多态性SLAF标记中，15,317个多态性SLAF可用于遗传图谱构建。整合后的图谱包含14,189个高质量SLAF，它们被归为23个遗传连锁群，总长度为2962.46厘摩，相邻标记之间的平均距离为0.21厘摩。此外，从已定位的标记中鉴定出了23,664个SNP。据我们所知，这是[物种名称未明确]的第一张遗传图谱。我们的结果进一步证明SLAF-seq是开发标记和构建高密度连锁图谱的一种非常有效的方法。本研究报道的SNP标记和遗传图谱应是未来研究中的宝贵资源。
