Nemes Z, Thomázy V
Department of Pathology, University Medical School of Debrecen, Hungary.
Cancer. 1988 Nov 1;62(9):1970-80. doi: 10.1002/1097-0142(19881101)62:9<1970::aid-cncr2820620917>3.0.co;2-v.
Interrelationships of immunologic and enzymatic markers of histiocytes have been studied in malignant neoplasms of histiocytic/monocytic origin and in differential diagnostically relevant, large cell non-Hodgkin's lymphomas. Cryostat sections required for demonstrating cell surface antigens by monoclonal antibodies are inadequate for studying cellular detail, enzymatic maturation by alpha-naphthyl acetate esterase (ANAE), and demonstrating the classical cytoplasmic markers of histiocytes like lysozyme, alpha-1-antitrypsin (AT), and alpha-1-antichymotrypsin (ACT). These markers have been compared in gently fixed and vacuum paraffin-embedded material. The reactivity for monoclonal anti-human monocyte 1 (Mo 1) has also been preserved by this method. Malignant histiocytosis (MH) is characterized by a heterogeneous cell population. The mature, ANAE-positive cells with macrophage morphology usually show a diffuse cytoplasmic positivity for AT and ACT. Lysozyme is moderately positive to negative in these cells, but it is more efficient than these markers in revealing smaller cells resembling monocytes by focal positivity in the cytoplasm. The expression of Factor XIIIa (F-XIIIa) is connected with the phagocytic activation of histiocytic cells. F-XIIIa positive cells usually form a minority of the neoplastic population in MH, but the large cytophagocytic marcophages are invariably positive. Reactive macrophages in large cell non-Hodgkin's lymphomas are characterized by a coexpression of ANAE, AT, ACT, lysozyme, F-XIIIa and Mo 1. Typical cases of true histiocytic lymphoma (THL) are made up of a homogeneous population showing the above mature, phagocytizing phenotype. In MH, Mo 1 and ANAE recognize different subpopulations. The reciprocal relation of these markers is an abnormal phenotypic feature. The results presented in this article prove the diagnostic value of ANAE and lysozyme in confirming the histiocytic differentiation of malignant cells. Monoclonal anti-human monocyte 1 is useful for identifying the immature component in MH. Factor XIIIa can be considered a functional marker of mature phagocytic histiocytes and an aid in the diagnosis of THL.
在组织细胞/单核细胞起源的恶性肿瘤以及鉴别诊断相关的大细胞非霍奇金淋巴瘤中，已对组织细胞的免疫和酶学标志物之间的相互关系进行了研究。通过单克隆抗体显示细胞表面抗原所需的低温切片，不足以研究细胞细节、α-萘乙酸酯酶（ANAE）的酶促成熟过程，以及显示组织细胞的经典细胞质标志物，如溶菌酶、α-1抗胰蛋白酶（AT）和α-1抗糜蛋白酶（ACT）。已在轻度固定和真空石蜡包埋的材料中对这些标志物进行了比较。通过这种方法，单克隆抗人单核细胞1（Mo 1）的反应性也得以保留。恶性组织细胞增多症（MH）的特征是细胞群体异质性。具有巨噬细胞形态的成熟、ANAE阳性细胞通常显示AT和ACT的弥漫性细胞质阳性。溶菌酶在这些细胞中呈中度阳性至阴性，但在通过细胞质中的局灶性阳性揭示类似单核细胞的较小细胞方面，它比这些标志物更有效。因子ⅩⅢa（F-XIIIa）的表达与组织细胞的吞噬激活有关。F-XIIIa阳性细胞在MH中通常占肿瘤细胞群体的少数，但大的吞噬性巨噬细胞总是呈阳性。大细胞非霍奇金淋巴瘤中的反应性巨噬细胞的特征是ANAE、AT、ACT、溶菌酶、F-XIIIa和Mo 1的共表达。真正的组织细胞淋巴瘤（THL）的典型病例由显示上述成熟吞噬表型的同质细胞群体组成。在MH中，Mo 1和ANAE识别不同的亚群。这些标志物的相互关系是一种异常的表型特征。本文给出的结果证明了ANAE和溶菌酶在确认恶性细胞的组织细胞分化方面的诊断价值。单克隆抗人单核细胞1有助于识别MH中的未成熟成分。因子ⅩⅢa可被视为成熟吞噬性组织细胞的功能标志物，并有助于THL的诊断。
