Hsu S M, Ho Y S, Hsu P L
Department of Pathology, University of Arkansas for Medical Science, Little Rock.
Am J Pathol. 1991 Jun;138(6):1389-404.
The authors determined the phenotypes of neoplastic cells in true histiocytic lymphoma and malignant histiocytosis by using a large panel of monoclonal antibodies and enzyme histochemistry procedures. Although the phenotypes overlapped slightly, the authors noted a distinct pattern in these tumors. The tumor cells of malignant histiocytosis generally expressed the monocyte markers CD11b, CD11c, CD14, and CD45, especially after induction with phorbol ester. In contrast, the tumor cells of true histiocytic lymphoma exhibited a marker expression very similar to that of Reed-Sternberg cells in Hodgkin's disease. These cells expressed markers CD30, 2H9, and 1A2, but rarely expressed CD11b, CD11c, CD14, or CD45. Regardless of their cytologic features, the tumor cells from both types of histiocytic lymphoma exhibited diffuse nonspecific esterase and acid phosphatase activities, and they expressed histiocyte markers CD15, CD68, LN5, 1E9, and M387 to varying degrees. The tumor cells from both lymphomas did not exhibit T- or B-cell markers, T-cell receptor or immunoglobulin gene rearrangements, or gene translation products, even when they were induced with phorbol ester. The phenotypic expression in these two histiocytic malignancies suggests that they are derived from different types of histiocytes, or from histiocytes in different stages of maturation or differentiation, or from histiocytes that have distinct mechanisms of tumorigenic transformation. The expression of circulating monocyte markers in malignant histiocytosis suggests that this tumor originates in monocytes or free histiocytes, whereas the phenotype of true histiocytic lymphoma is compatible with an origin in fixed histiocytes, which generally are devoid of the monocyte markers CD11b and CD14.
作者通过使用大量单克隆抗体和酶组织化学方法，确定了真性组织细胞淋巴瘤和恶性组织细胞增多症中肿瘤细胞的表型。尽管这些表型略有重叠，但作者注意到这些肿瘤存在明显的模式。恶性组织细胞增多症的肿瘤细胞通常表达单核细胞标志物CD11b、CD11c、CD14和CD45，尤其是在用佛波酯诱导后。相比之下，真性组织细胞淋巴瘤的肿瘤细胞表现出与霍奇金病里德-斯腾伯格细胞非常相似的标志物表达。这些细胞表达标志物CD30、2H9和1A2，但很少表达CD11b、CD11c、CD14或CD45。无论其细胞学特征如何，两种组织细胞淋巴瘤的肿瘤细胞均表现出弥漫性非特异性酯酶和酸性磷酸酶活性，并且它们不同程度地表达组织细胞标志物CD15、CD68、LN5、1E9和M387。两种淋巴瘤的肿瘤细胞均未表现出T或B细胞标志物、T细胞受体或免疫球蛋白基因重排，或基因翻译产物，即使在用佛波酯诱导时也是如此。这两种组织细胞恶性肿瘤中的表型表达表明，它们源自不同类型的组织细胞，或源自成熟或分化不同阶段的组织细胞，或源自具有不同致瘤转化机制的组织细胞。恶性组织细胞增多症中循环单核细胞标志物的表达表明，这种肿瘤起源于单核细胞或游离组织细胞，而真性组织细胞淋巴瘤的表型与固定组织细胞的起源相符，固定组织细胞通常缺乏单核细胞标志物CD11b和CD14。
