Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
Howard Hughes Medical Institute, Seattle, Washington, USA.
Nature. 2017 Oct 19;550(7676):345-353. doi: 10.1038/nature24286. Epub 2017 Oct 11.
This review commemorates the 40th anniversary of DNA sequencing, a period in which we have already witnessed multiple technological revolutions and a growth in scale from a few kilobases to the first human genome, and now to millions of human and a myriad of other genomes. DNA sequencing has been extensively and creatively repurposed, including as a 'counter' for a vast range of molecular phenomena. We predict that in the long view of history, the impact of DNA sequencing will be on a par with that of the microscope.
本文回顾了 DNA 测序 40 年的发展历程，测序技术已历经多次变革，从最初的几 kb 发展到人类基因组，再到现在的上百万个人类基因组和不计其数的其他物种基因组。DNA 测序技术得到了广泛而富有创意的应用，包括作为各种分子现象的“计数器”。我们预测，从历史的长远角度来看，DNA 测序的影响将与显微镜相媲美。
