Hong Yang Hee, Lee Hyun-Sun, Jung Eun Young, Han Sung-Hee, Park Yooheon, Suh Hyung Joo
Department of Beauty Art, Suwon Women's University, Suwon, Republic of Korea.
Agency for Korea National Food Cluster, Iksan Jeonbuk, Republic of Korea.
J Ginseng Res. 2017 Oct;41(4):456-462. doi: 10.1016/j.jgr.2016.07.007. Epub 2016 Aug 6.
Abnormal activation of matrix metalloproteinases (MMPs) plays an important role in UV-induced wrinkle formation, which is a major dermatological problem. This formation occurs due to the degeneration of the extracellular matrix (ECM). In this study, we investigated the cutaneous photoprotective effects of Ultraflo L treated ginseng leaf (UTGL) in hairless mice.
SKH-1 hairless mice (6 weeks of age) were randomly divided into four groups (8 mice/group). UTGL formulation was applied topically to the skin of the mice for 10 weeks. The normal control group received nonvehicle and was not irradiated with UVB. The UV control (UVB) group received nonvehicle and was exposed to gradient-UVB irradiation. The groups (GA) receiving topical application of UTGL formulation were subjected to gradient-UVB irradiation on 0.5 mg/cm [GA-low (GA-L)] and 1.0 mg/cm [(GA-high (GA-H)] of dorsal skin area, respectively.
We found that topical treatment with UTGL attenuated UVB-induced epidermal thickness and impairment of skin barrier function. Additionally, UTGL suppressed the expression of MMP-2, -3, and -13 induced by UVB irradiation. Our results show that topical application of UTGL protects the skin against UVB-induced damage in hairless mice and suggest that UTGL can act as a potential agent for preventing and/or treating UVB-induced photoaging.
UTGL possesses sunscreen properties and may exhibit photochemoprotective activities inside the skin of mice. Therefore, UTGL could be used as a potential therapeutic agent to protect the skin against UVB-induced photoaging.
基质金属蛋白酶（MMPs）的异常激活在紫外线诱导的皱纹形成中起重要作用，而皱纹形成是一个主要的皮肤病学问题。这种形成是由于细胞外基质（ECM）的退化所致。在本研究中，我们调查了经Ultraflo L处理的人参叶（UTGL）对无毛小鼠的皮肤光保护作用。
将6周龄的SKH-1无毛小鼠随机分为四组（每组8只小鼠）。将UTGL制剂局部应用于小鼠皮肤10周。正常对照组接受无载体处理且未接受UVB照射。紫外线对照组（UVB）接受无载体处理并暴露于梯度UVB照射。局部应用UTGL制剂的组（GA）分别在背部皮肤面积为0.5 mg/cm [GA-低剂量组（GA-L）]和1.0 mg/cm [GA-高剂量组（GA-H）]时接受梯度UVB照射。
我们发现局部应用UTGL可减轻UVB诱导的表皮厚度增加和皮肤屏障功能受损。此外，UTGL抑制了UVB照射诱导的MMP-2、-3和-13的表达。我们的结果表明，局部应用UTGL可保护无毛小鼠皮肤免受UVB诱导的损伤，并表明UTGL可作为预防和/或治疗UVB诱导的光老化的潜在药物。
UTGL具有防晒特性，可能在小鼠皮肤内表现出光化学保护活性。因此，UTGL可作为一种潜在的治疗药物来保护皮肤免受UVB诱导的光老化。
