Bezerra Júnior Rosivaldo Quirino, Eloy Ângela Maria Xavier, Furtado João Ricardo, Pinheiro Raymundo Rizaldo, Andrioli Alice, Moreno Frederico Bruno, Pinto Lobo Marina Duarte, Monteiro-Moreira Ana Cristina O, de Azevedo Moreira Renato, Farias Pinto Tatiana Maria, da Silva Teixeira Maria Fátima
Laboratory of Virology (LABOVIR), Ceará State University (UECE), Fortaleza, Ceará, Brazil.
Brazilian Agricultural Research Corporation (EMBRAPA Goats and Sheep), PO. BOX 145, Sobral, 62010-970, Brazil.
Trop Anim Health Prod. 2018 Jan;50(1):43-48. doi: 10.1007/s11250-017-1398-1. Epub 2017 Oct 12.
The caprine arthrite encephalite (CAE) is a disease that affects especially dairy goat. The virus shows compartmentalization features, that allows it to hide at certain times during the course of the disease, making it difficult to control. The present study was conducted to identify the major seminal plasma protein profile of goats infected by CAE and its associations with seroconversion using Western blotting. Two groups containing five males each, were used in this experiment. The first group was composed by seropositive animals and the control by seronegative confirmed by Western blotting and PCR. The semen was collected through artificial vagina and after that, two-dimensional electrophoresis and MALDI-TOF MS were used. Seventy-five spots were identified in the goat seminal plasma gels, equivalent to 13 different proteins with more expression. The similar proteins found in both groups and related to reproduction were spermadhesin Z13-like, bodhesin and bodhesin-2, Lipocalin, protein PDC-109-like, and albumin. In infected goats, proteases such as arisulfatase A have been identified, whose function probably is related to metabolism control of sulfatides, involved to virus control. The other ones were bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase, cathepsin F isoform X1, disintegrin and metalloproteinase domain-containing protein 2-like isoform X1, clusterin, carbonic anhydrase 2, electron transfer flavoprotein subunit beta, and epididymal secretory glutathione peroxidase. The results of this study show the reaction of the innate immune system against chronic infection of goats by CAE.
山羊关节炎脑炎（CAE）是一种主要影响奶山羊的疾病。该病毒具有分区化特征，这使得它在疾病过程中的某些时候能够隐藏起来，从而难以控制。本研究旨在通过蛋白质免疫印迹法鉴定感染CAE的山羊主要精浆蛋白谱及其与血清转化的关联。本实验使用了两组，每组五只雄性山羊。第一组由血清阳性动物组成，对照组由经蛋白质免疫印迹法和聚合酶链反应确认的血清阴性动物组成。精液通过人工阴道采集，之后使用二维电泳和基质辅助激光解吸电离飞行时间质谱。在山羊精浆凝胶中鉴定出75个斑点，相当于13种表达量更高的不同蛋白质。两组中发现的与生殖相关的相似蛋白质有精子黏附素Z13样蛋白、博德辛和博德辛-2、脂质运载蛋白、PDC-109样蛋白和白蛋白。在感染山羊中，已鉴定出诸如芳基硫酸酯酶A等蛋白酶，其功能可能与硫苷脂的代谢控制有关，参与病毒控制。其他的有双功能ATP依赖性二羟基丙酮激酶/FAD-AMP裂解酶、组织蛋白酶F同工型X1、含解整合素和金属蛋白酶结构域的蛋白2样同工型X1、聚集素、碳酸酐酶2、电子传递黄素蛋白亚基β和附睾分泌型谷胱甘肽过氧化物酶。本研究结果显示了先天免疫系统对山羊CAE慢性感染的反应。
