Mansfield Jillian M, Herrmann Paul, Jesionowski Amy M, Vickerman M Margaret
Department of Oral Biology, School of Dental Medicine, University at Buffalo, Buffalo, NY 14214, USA.
J Med Microbiol. 2017 Nov;66(11):1635-1639. doi: 10.1099/jmm.0.000613. Epub 2017 Oct 12.
Streptococcus gordonii produces a pheromone heptapeptide, s.g.cAM373, which induces a conjugative mating response in Enterococcus faecalis cells carrying the responsive plasmid, pAM373. We investigated the extent of this intergeneric signaling on DNA acquisition by streptococcal species likely to cohabit oral biofilms. E. faecalis/pAM373/pAMS470 cells were incubated with synthetic s.g.cAM373, reverse peptide s.g.cAM373-R, or peptide-free medium and examined for their abilities to transfer plasmid DNA to streptococcal species in the presence of DNase. Preinduction of E. faecalis donors with s.g.cAM373 resulted in transconjugation frequencies in non-pheromone producing strains of Streptococcus mutans, Streptococcus sanguinis, Streptococcus anginosus, and Streptococcus suis that were significantly higher than frequencies when donors were preincubated with s.g.cAM373-R or medium alone. Peptide-mediated communication between commensal streptococci and E. faecalis carrying pheromone-responsive plasmids may facilitate conjugative DNA transfer to bystander species, and influence the reservoir of antibiotic resistance determinants of enterococcal origin in the oral metagenome.
戈登氏链球菌产生一种信息素七肽，即s.g.cAM373，它能在携带响应质粒pAM373的粪肠球菌细胞中诱导接合交配反应。我们研究了这种种间信号传导对可能共同存在于口腔生物膜中的链球菌物种获取DNA的影响程度。将粪肠球菌/ pAM373 / pAMS470细胞与合成的s.g.cAM373、反向肽s.g.cAM373-R或无肽培养基一起孵育，并检测它们在存在DNA酶的情况下将质粒DNA转移到链球菌物种的能力。用s.g.cAM373对粪肠球菌供体进行预诱导，导致变形链球菌、血链球菌、咽峡炎链球菌和猪链球菌等不产生信息素的菌株中的转接合频率显著高于供体仅与s.g.cAM373-R或培养基预孵育时的频率。共生链球菌与携带信息素响应质粒的粪肠球菌之间的肽介导通讯可能促进接合性DNA向旁观者物种的转移，并影响口腔宏基因组中肠球菌来源的抗生素抗性决定因素的储存库。
