Bernhard Christoph, Roeters Steven J, Franz Johannes, Weidner Tobias, Bonn Mischa, Gonella Grazia
Max Planck Institute for Polymer Research, Ackermannweg 10, D-55128 Mainz, Germany.
Phys Chem Chem Phys. 2017 Oct 25;19(41):28182-28188. doi: 10.1039/c7cp05445a.
Development of new materials for drug delivery and biosensing requires the fine-tuning of interfacial properties. We report here the influence of the poly(ethylene glycol) (PEG) grafting density in model phospholipid monolayers on the adsorption behavior of bovine serum albumin and human fibrinogen, not only with respect to the amount of adsorbed protein, but also its orientational ordering on the surface. As expected, with increasing interfacial PEG density, the amount of adsorbed protein decreases up to the point where complete protein repellency is reached. However, at intermediate concentrations, the net orientation of adsorbed fibrinogen is highest. The different proteins respond differently to PEG, not only in the amount of protein adsorbed, but also in the manner that proteins adsorb. The results show that for specific cases, tuning the interfacial PEG concentration allows to guide the protein adsorption configuration, a feature sought after in materials for both biosensing and biomedical applications.
开发用于药物递送和生物传感的新材料需要对界面性质进行微调。我们在此报告了模型磷脂单层中聚乙二醇（PEG）接枝密度对牛血清白蛋白和人纤维蛋白原吸附行为的影响，不仅涉及吸附蛋白的量，还包括其在表面的取向有序性。正如预期的那样，随着界面PEG密度的增加，吸附蛋白的量会减少，直至达到完全排斥蛋白的程度。然而，在中间浓度时，吸附的纤维蛋白原的净取向最高。不同的蛋白质对PEG的反应不同，不仅体现在吸附蛋白的量上，还体现在蛋白质吸附的方式上。结果表明，在特定情况下，调节界面PEG浓度可以引导蛋白质吸附构型，这是生物传感和生物医学应用材料所追求的一个特性。
