Traore I M A, Zohoncon T M, Ndo O, Djigma F W, Obiri-Yeboah D, Compaore T R, Guigma S P, Yonli A T, Traore G, Ouedraogo P, Ouedraogo C M R, Traore Y, Simpore J
Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Health District of Orodara, Burkina Faso.
Pak J Biol Sci. 2016;19(7):306-311. doi: 10.3923/pjbs.2016.306.311.
Cervical cancer usually occurs several years after persistent infection with oncogenic or high-risk human papillomavirus. The objective of this study was to determine carriage of 14 genotypes of high-risk human papillomavirus among women at Orodara and then characterize the genotypes found in these women.
From June to July 2015, 120 women from the general population were recruited in the health district of Orodara. They voluntarily agreed to participate in the study. Endocervical samples were taken from these women prior to screening for precancerous lesions by visual inspection with acetic acid and lugol's iodine. Identification of high-risk human papillomavirus genotype was done using real-time PCR.
High-risk human papillomavirus prevalence was 38.3% and the most common genotypes were HPV 52 (25.4%), HPV 33 (20.6%) and HPV 59 (11.1%). The HPV 66 was also identified with a prevalence of 9.5%.
The HPV 16 and HPV 18 which are frequently associated with cancer worldwide were not found among the most frequent oncogenic HPV in women in Orodara.
宫颈癌通常在致癌性或高危型人乳头瘤病毒持续感染数年之后发生。本研究的目的是确定奥罗达拉地区女性中14种高危型人乳头瘤病毒基因型的携带情况,然后对这些女性中发现的基因型进行特征分析。
2015年6月至7月,在奥罗达拉卫生区招募了120名普通女性。她们自愿同意参与本研究。在通过醋酸和卢戈氏碘目视检查筛查癌前病变之前,从这些女性中采集宫颈管样本。使用实时聚合酶链反应对高危型人乳头瘤病毒基因型进行鉴定。
高危型人乳头瘤病毒的患病率为38.3%,最常见的基因型是HPV 52(25.4%)、HPV 33(20.6%)和HPV 59(11.1%)。HPV 66的患病率也为9.5%。
在奥罗达拉地区女性中最常见的致癌性人乳头瘤病毒中未发现全球范围内经常与癌症相关的HPV 16和HPV 18。