多哥洛美地区女性中高危型人乳头瘤病毒（HR-HPV）的分子特征。
Molecular characterization of high-risk human papillomavirus (HR-HPV) in women in Lomé, Togo.
机构信息
Molecular Biology and Genetics Laboratory (LABIOGENE), Department of Biochemistry and Microbiology, University JOSEPH KI-ZERBO, P.O. Box 7021, Ouagadougou 03, Burkina Faso.
Pietro Annigoni Biomolecular Research Centre (CERBA), P.O. Box 364, Ouagadougou 01, Burkina Faso.
出版信息
BMC Infect Dis. 2021 Mar 19;21(1):278. doi: 10.1186/s12879-021-05956-5.
BACKGROUND
The causative agent of cervical cancer referred to as Human papillomavirus (HPV) remains a real public health problem. Many countries in West Africa, such as Togo have no data on the high-risk HPV (HR-HPV) infection and genotypes distribution. In order to fill the knowledge gap in the field in Togo, the main objective of this study was to determine the prevalence of pre-cancerous lesions of the cervix and HR-HPV genotypes among Togolese women.
METHODS
Samples were collected from 240 women by introducing a swab in the cervix. Then, the screening of precancerous cervical lesions using the visual inspection with acetic acid and lugol (VIA / VIL) was conducted. The HR-HPV genotypes were characterised by real-time multiplex PCR.
RESULTS
Out of 240 women recruited, 128 (53.3%) were infected by HR-HPV. The most common genotypes were HPV 56 (22.7%), followed by HPV 51 (20.3%), HPV 31 (19.5%), HPV 52 (18.8%) and HPV 35 (17.2%). The least common genotypes were HPV 33 (2.3%) and HPV 16 (2.3%). Among the women, 1.3% (3/240) were positive to VIA/VIL.
CONCLUSION
This study allowed HR-HPV genotypes to be characterised for the first time in Lomé, Togo. This will help in mapping the HR-HPV genotypes in West Africa.
背景
宫颈癌的病原体人乳头瘤病毒（HPV）仍然是一个真正的公共卫生问题。许多西非国家，如多哥，没有关于高危型 HPV（HR-HPV）感染和基因型分布的数据。为了填补多哥在该领域的知识空白，本研究的主要目的是确定多哥妇女的宫颈癌前病变和 HR-HPV 基因型的流行率。
方法
通过向宫颈内插入拭子从 240 名妇女中采集样本。然后，通过醋酸和卢戈氏碘液（VIA/VIL）目视检查进行宫颈癌前病变的筛查。通过实时多重 PCR 对 HR-HPV 基因型进行特征描述。
结果
在招募的 240 名妇女中，有 128 名（53.3%）感染了 HR-HPV。最常见的基因型是 HPV 56（22.7%），其次是 HPV 51（20.3%）、HPV 31（19.5%）、HPV 52（18.8%）和 HPV 35（17.2%）。最不常见的基因型是 HPV 33（2.3%）和 HPV 16（2.3%）。在这些妇女中，有 1.3%（3/240）对 VIA/VIL 呈阳性。
结论
本研究首次在多哥对 HR-HPV 基因型进行了特征描述。这将有助于绘制西非的 HR-HPV 基因型图谱。
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