Tovo Sessi Frida, Zohoncon Théodora Mahoukèdè, Dabiré Amana Metuor, Ilboudo Régine, Tiemtoré Rahimatou Yasmine, Obiri-Yeboah Dorcas, Yonli Albert Théophane, Dovo Essi Etonam, Ouédraogo Rogomenoma Alice, Ouattara Abdoul Karim, Sorgho Pegdwende Abel, Ouermi Djénéba, Djigma Florencia Wendkuuni, Ouédraogo Charlemagne, Sangaré Lassana, Simpore Jacques
Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE), Ouagadougou BP 7021, Burkina Faso.
Biomolecular Research Center Pietro Annigoni (CERBA), Ouagadougou BP 364, Burkina Faso.
Trop Med Infect Dis. 2021 May 27;6(2):90. doi: 10.3390/tropicalmed6020090.
Viral and bacterial infections represent an occupational risk for female sex workers. This study aimed at determining HPV coinfection with genital pathogens among female sex workers in West and Central Africa and identifying antibiotic resistance genes. A total of 182 samples from female sex workers were analyzed by real-time PCR and classic PCR. For the molecular diagnosis of HPV, the real-time multiplex amplification kit "HPV Genotypes 14 Real-TM Quant" from SACACE Biotechnologies, detecting 14 high-risk HPV genotypes, was used, while for other pathogens, the real-time multiplex amplification kit /// Real-TM, allowing their simultaneous detection, was used. The women were aged 17-50 years with an average age of 27.12 ± 6.09 years. The pathogens identified were HPV 54.94% (100/120), (13.74%), (11.54%) and (11.54%). The most common HPV genotypes were HPV68, HPV38 and HPV52. The antibiotic resistance genes identified were 24.00%, 22.00%, 17.00%, 13.00% and 1.00%. This study revealed the presence of various HPV genotypes associated with other pathogens with problems of antibiotic resistance among sex workers of West and Central African origin working in Ouagadougou.
病毒和细菌感染是女性性工作者面临的职业风险。本研究旨在确定西非和中非女性性工作者中HPV与生殖病原体的合并感染情况，并鉴定抗生素耐药基因。通过实时PCR和经典PCR对总共182份女性性工作者的样本进行了分析。对于HPV的分子诊断，使用了SACACE生物技术公司的实时多重扩增试剂盒“HPV Genotypes 14 Real-TM Quant”，该试剂盒可检测14种高危HPV基因型，而对于其他病原体，则使用了实时多重扩增试剂盒/// Real-TM，可同时检测这些病原体。这些女性年龄在17至50岁之间，平均年龄为27.12±6.09岁。鉴定出的病原体为HPV 54.94%（100/120），（13.74%），（11.54%）和（11.54%）。最常见的HPV基因型为HPV68、HPV38和HPV52。鉴定出的抗生素耐药基因为24.00%，22.00%，17.00%，13.00%和1.00%。本研究揭示了在瓦加杜古工作的西非和中非裔性工作者中存在与其他病原体相关的多种HPV基因型以及抗生素耐药问题。
