Technical and Macromolecular Chemistry, Paderborn University, Warburger Str. 100, 33098, Paderborn, Germany.
B CUBE - Center for Molecular Bioengineering, Technische Universität Dresden, Arnoldstr. 18, 01307, Dresden, Germany.
Small. 2017 Nov;13(44). doi: 10.1002/smll.201702100. Epub 2017 Oct 12.
The stability of DNA origami nanostructures under various environmental conditions constitutes an important issue in numerous applications, including drug delivery, molecular sensing, and single-molecule biophysics. Here, the effect of Na and Mg concentrations on DNA origami stability is investigated in the presence of urea and guanidinium chloride (GdmCl), two strong denaturants commonly employed in protein folding studies. While increasing concentrations of both cations stabilize the DNA origami nanostructures against urea denaturation, they are found to promote DNA origami denaturation by GdmCl. These inverse behaviors are rationalized by a salting-out of Gdm to the hydrophobic DNA base stack. The effect of cation-induced DNA origami denaturation by GdmCl deserves consideration in the design of single-molecule studies and may potentially be exploited in future applications such as selective denaturation for purification purposes.
在各种环境条件下，DNA 折纸纳米结构的稳定性是许多应用的重要问题，包括药物输送、分子传感和单分子生物物理学。在这里，研究了在存在尿素和胍盐酸盐（GdmCl）的情况下，Na 和 Mg 浓度对 DNA 折纸稳定性的影响，尿素和 GdmCl 是常用于蛋白质折叠研究的两种强变性剂。虽然两种阳离子的浓度增加都能稳定 DNA 折纸纳米结构抵抗尿素变性，但它们被发现能促进 GdmCl 诱导的 DNA 折纸变性。这种相反的行为可以通过 Gdm 盐析到疏水性 DNA 碱基堆叠来合理化。GdmCl 诱导的阳离子引起的 DNA 折纸变性的影响在单分子研究的设计中值得考虑，并且可能在未来的应用中得到利用，例如选择性变性用于纯化目的。
