el Monem A M, Saad M, Lindquist B L, Lee P C, Abud R, Merrick J M, Lebenthal E
International Institute for Infant Nutrition and Gastrointestinal Disease, Children's Hospital, Buffalo, NY 14222.
Pediatr Res. 1988 Oct;24(4):508-11. doi: 10.1203/00006450-198810000-00019.
The adherence of radiolabeled fimbriated (S 7471 OF) and nonfimbriated (S 7471 N) Salmonella typhimurium to small intestinal rat enterocytes was examined during postnatal development. The fimbriated strain invariably adhered in higher numbers than the nonfimbriated strain during all periods of development. The capability of enterocytes to bind Salmonella increased significantly during postnatal development and reached adult levels at weaning time (21 days of age). Bacterial adherence to enterocytes was similar if the cells were isolated from the proximal or the distal small intestine. Early weaning of pups did not affect the capability of enterocytes to bind Salmonella. Pretreatment of isolated enterocytes from adult animals with rat's milk before exposure to Salmonella had no effect on the level of bacteria that adhered per enterocyte. Conversely, pretreatment of Salmonella with rats' milk before binding to enterocytes from adult animals also did not alter the level of bacteria adhered per enterocyte. These results suggest an age-dependent, postnatal development of available receptors for S. typhimurium on rat enterocytes. The acquisition of these receptors is not affected by mother's milk and is unaltered by early weaning.
在出生后的发育过程中，研究了放射性标记的有菌毛（S 7471 OF）和无菌毛（S 7471 N）鼠伤寒沙门氏菌对大鼠小肠肠上皮细胞的黏附情况。在发育的各个阶段，有菌毛菌株的黏附数量始终高于无菌毛菌株。在出生后的发育过程中，肠上皮细胞结合沙门氏菌的能力显著增强，并在断奶时（21日龄）达到成年水平。如果从近端或远端小肠分离细胞，细菌对肠上皮细胞的黏附情况相似。幼崽提前断奶并不影响肠上皮细胞结合沙门氏菌的能力。在将成年动物分离的肠上皮细胞暴露于沙门氏菌之前，用大鼠乳汁进行预处理对每个肠上皮细胞黏附的细菌水平没有影响。相反，在沙门氏菌与成年动物的肠上皮细胞结合之前，用大鼠乳汁进行预处理也不会改变每个肠上皮细胞黏附的细菌水平。这些结果表明，大鼠肠上皮细胞上鼠伤寒沙门氏菌可用受体在出生后呈年龄依赖性发育。这些受体的获得不受母乳影响，提前断奶也不会改变。
