Lindquist B L, Lebenthal E, Lee P C, Stinson M W, Merrick J M
International Institute for Infant Nutrition and Gastrointestinal Disease, Children's Hospital, Buffalo, New York 14222.
Infect Immun. 1987 Dec;55(12):3044-50. doi: 10.1128/iai.55.12.3044-3050.1987.
The adherence of radiolabeled Salmonella typhimurium to freshly isolated enterocytes of rats was studied. The results established that type 1 fimbriated strains adhered in significantly higher numbers than did related nonfimbriated strains. Adherence was inhibited by D-mannose and methyl alpha-D-mannoside. Results of kinetic studies indicated that adherence was biphasic; the number of bacteria that adhered per enterocyte remained constant for approximately 20 min and then increased rapidly under the assay conditions. The second phase was associated with structural damage to the enterocytes. The addition of chloramphenicol did not prevent the initial attachment of bacteria to enterocytes but did prevent the second phase. Viable and nonviable bacterial cells adhered to enterocytes, but only viable bacteria were destructive. Freshly isolated enterocytes (trypan blue impermeable) and enterocytes stored overnight (trypan blue permeable) were infected by viable S. typhimurium in a similar manner, suggesting that metabolic activity of the host cell was of less consequence than metabolic activity of the bacterial cells. A model for the role of mannose-sensitive fimbriae as a virulence factor is proposed.
研究了放射性标记的鼠伤寒沙门氏菌对新鲜分离的大鼠肠上皮细胞的黏附情况。结果表明，1型菌毛菌株的黏附数量明显高于相关的无菌毛菌株。D-甘露糖和α-D-甲基甘露糖苷可抑制黏附。动力学研究结果表明，黏附是双相的；每个肠上皮细胞黏附的细菌数量在约20分钟内保持恒定，然后在测定条件下迅速增加。第二阶段与肠上皮细胞的结构损伤有关。添加氯霉素并不能阻止细菌最初与肠上皮细胞的附着，但能阻止第二阶段。活的和死的细菌细胞都能黏附到肠上皮细胞，但只有活细菌具有破坏性。新鲜分离的肠上皮细胞（台盼蓝拒染）和过夜储存的肠上皮细胞（台盼蓝透染）被活的鼠伤寒沙门氏菌以类似方式感染，这表明宿主细胞的代谢活性不如细菌细胞的代谢活性重要。提出了一个关于甘露糖敏感菌毛作为毒力因子作用的模型。
