Yadav Namrata, Srivastava Pradeep
School of Biochemical Engineering, IIT (BHU), Varanasi, 221005, India.
Heliyon. 2019 May 9;5(5):e01633. doi: 10.1016/j.heliyon.2019.e01633. eCollection 2019 May.
A promising route towards bone tissue engineering is made by the raw materials of the composite which mimics properties of the extracellular matrix components. Herein, the favourable human origin bone cells were seeded on the scaffold types to investigate the best co-culture system. The same has been achieved after the synthesis and characterization of gelatin/hydroxyapatite composites infused with chitosan for their calcium to phosphate (Ca/P) ratio by SEM-EDX. Also, biodegradation and bio-mineralization determined after immersing in lysozyme and SBF respectively. Uni-axial Compressive Strength (UCS), porosity, qualitative to quantitative phase development by XRD and FTIR were evaluated. The human bone cell-seeded composite was tested by flow cytometry, CLSM, SEM and DSC. This study statistically signified the human mesenchymal stem cell (hM) derived bone cell as potential raw material for minor to severe bone related tissue regenerative studies.
一种有前景的骨组织工程途径是由模仿细胞外基质成分特性的复合材料的原材料构成。在此，将良好的人类来源骨细胞接种在支架类型上，以研究最佳的共培养系统。通过扫描电子显微镜 - 能谱仪（SEM - EDX）对注入壳聚糖的明胶/羟基磷灰石复合材料的钙磷（Ca/P）比进行合成和表征后，也实现了同样的目标。此外，分别在溶菌酶和模拟体液（SBF）中浸泡后测定了生物降解和生物矿化情况。评估了单轴抗压强度（UCS）、孔隙率、通过X射线衍射（XRD）和傅里叶变换红外光谱（FTIR）进行的定性到定量相发展。通过流式细胞术、共聚焦激光扫描显微镜（CLSM）、扫描电子显微镜（SEM）和差示扫描量热法（DSC）对接种了人类骨细胞的复合材料进行了测试。这项研究从统计学上表明，人类间充质干细胞（hM）来源的骨细胞作为从轻度到重度骨相关组织再生研究的潜在原材料。
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