低表达诱导口腔癌细胞的增殖和侵袭，通过促进 FAP 的表达。
Low expression of induced the proliferation and invasion of oral cancer via promoting the expression of FAP.
机构信息
Department of Oncology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, P.R. China.
Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, P.R. China
出版信息
Biosci Rep. 2018 Jan 25;38(1). doi: 10.1042/BSR20171027. Print 2018 Feb 28.
The study aimed at investigating the effects of on the biological functions of oral cancer cells and figuring out the potential mechanism. We first verified the low expression of and high expression of FAP ( fibroblast activation protein α) in oral cancerous tissues and their negative correlation. Then, the target relationship between and FAP was validated by dual luciferase reporter assay and biotin-coupled miRNA pulldown assay. After transfection in Tca-8113 cells and SCC-15 cells, MTT, colony formation, Transwell, and wound healing assays were performed to investigate how and FAP adjusted propagation, invasiveness, and migration, respectively. Mounting evidence supported that directly targetted FAP and suppressed its expression in oral cavity cancer cells (OSCCs). By suppressing FAP expression, significantly inhibited cell propagation, migration, and invasion. Therefore, might be a new therapeutic target for oral cancer treatment.
本研究旨在探讨在口腔癌细胞的生物学功能的影响，并找出潜在的机制。我们首先验证了在口腔癌组织中的低表达和高水平的表达 FAP（成纤维细胞激活蛋白 α）及其负相关。然后，通过双荧光素酶报告基因检测和生物素偶联 miRNA 下拉实验验证了的靶关系和 FAP。转染 Tca-8113 细胞和 SCC-15 细胞后，通过 MTT、集落形成、Transwell 和划痕愈合实验分别研究了和 FAP 如何调节增殖、侵袭和迁移。越来越多的证据表明，直接靶向 FAP，并抑制其在口腔癌细胞（OSCCs）中的表达。通过抑制 FAP 的表达，显著抑制细胞的增殖、迁移和侵袭。因此，可能是治疗口腔癌的新的治疗靶点。
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