Pang Long, Dai Chunqiu, Bi Long, Guo Zhongshang, Fan Junjun
The 3rd Orthopedic Department of General Hospital, Ningxia Medical University, Ningxia, 750004, China.
Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University, No. 15 West Changle road, Xi'an, 710032, China.
Nanoscale Res Lett. 2017 Oct 12;12(1):564. doi: 10.1186/s11671-017-2317-0.
In recent years, graphene (G) and graphene oxide (GO) nanoparticles have begun to be applied in surgical implant surface modification. However, biosafety and antibacterial ability of G and GO are still unclear. In this study, the biosafety of G and GO in vitro was evaluated by co-culture with bone marrow mesenchymal stem cells (BMSCs) and biosafety in vivo was observed by implanting materials into mice muscle tissue. Biosafety results showed that 10 μg/ml was the safety critical concentration for G and GO. When the concentration was more than 10 μg/ml, the cytotoxicity of G and GO showed a dose-dependent manner.Antibacterial results showed that G presented the antibacterial ability with the concentration equal to and more than 100 μg/ml; GO presented the antibacterial ability with the concentration equal to and more than 50 μg/ml. The antibacterial effect of G and GO were in a dose-dependent manner in vitro.The GO or G concentration between 50 and 100 μg/ml may be the better range to keep the balance of cytotoxicity and antibacterial ability. Our study reveals that G and GO have potential to be used in clinic with good biosafety and antibacterial properties in a certain concentration range.
近年来，石墨烯（G）和氧化石墨烯（GO）纳米颗粒已开始应用于外科植入物表面改性。然而，G和GO的生物安全性和抗菌能力仍不明确。在本研究中，通过与骨髓间充质干细胞（BMSCs）共培养评估了G和GO在体外的生物安全性，并通过将材料植入小鼠肌肉组织观察了其在体内的生物安全性。生物安全性结果表明，10μg/ml是G和GO的安全临界浓度。当浓度超过10μg/ml时，G和GO的细胞毒性呈剂量依赖性。抗菌结果表明，G在浓度等于及高于100μg/ml时具有抗菌能力；GO在浓度等于及高于50μg/ml时具有抗菌能力。G和GO的抗菌作用在体外呈剂量依赖性。50至100μg/ml之间的GO或G浓度可能是保持细胞毒性和抗菌能力平衡的较好范围。我们的研究表明，G和GO在一定浓度范围内具有良好的生物安全性和抗菌特性，有潜力应用于临床。
