Department of Polymer Engineering, Faculty of Engineering, Golestan University, Gorgan, Iran.
Department of Polymer Engineering, Faculty of Engineering, Golestan University, Gorgan, Iran.
Carbohydr Polym. 2019 Dec 1;225:115220. doi: 10.1016/j.carbpol.2019.115220. Epub 2019 Aug 20.
The main objective of this work was to find a way to increase the bio-applicability of graphene oxide (GO) nanoparticles. In this way, various kinds of graphene oxide-chitosan (GO-CS) nano-hybrids were synthesized through attachment of different kinds of chitosan (CS) structures with GO. Subsequently, they were assessed in terms of structural characterization, antibacterial activity and cytotoxicity to obtain a hybrid structure representing the highest bactericidal and biocompatibility performance. Our results revealed that the single-layer GO and also three different kinds of GO-CS nano-hybrid structures (pristine powder, spherical and nano-fibrilar network structures) were successfully synthesized. Antibacterial activity results indicated superior antibacterial activity of nano-hybrids compared to the pure GO. In addition, it was observed that the attachment of CS to GO interestingly reduced the cytotoxicity effect of GO and even caused cell proliferation in some samples. Furthermore, the antibacterial and bio-safety properties of different hybrids were compared and suggestive mechanisms for their particular performances were proposed.
本工作的主要目的是寻找一种提高氧化石墨烯（GO）纳米粒子生物适用性的方法。为此，通过将不同结构的壳聚糖（CS）与 GO 连接，合成了各种氧化石墨烯-壳聚糖（GO-CS）纳米杂化物。随后，对它们进行了结构表征、抗菌活性和细胞毒性评估，以获得一种具有最高杀菌和生物相容性性能的杂化结构。我们的结果表明，成功合成了单层 GO 以及三种不同结构的 GO-CS 纳米杂化物（原始粉末、球形和纳米纤维网络结构）。抗菌活性结果表明，纳米杂化物比纯 GO 具有更好的抗菌活性。此外，观察到 CS 与 GO 的结合有趣地降低了 GO 的细胞毒性作用，甚至在一些样品中引起了细胞增殖。此外，还比较了不同杂化物的抗菌和生物安全性特性，并提出了它们特殊性能的可能机制。
